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Anti-CRLR/CALCRL (extracellular) Antibody

Calcitonin Receptor-Like Receptor, Calcitonin Gene-Related Peptide Type 1 Receptor, CRL, CGRPR
Cat #: ACR-060
Alternative Name Calcitonin Receptor-Like Receptor, Calcitonin Gene-Related Peptide Type 1 Receptor, CRL, CGRPR
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CNVNTHEKVKTALN, corresponding to amino acid residues 127 - 140 of human Calcitonin Receptor-Like Receptor (Accession Q16602). Extracellular, N-terminus.
Accession (Uniprot) Number Q16602
Gene ID 10203
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat – 12/14 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ih*, ip*, lci*
Western blot
  • Western blot analysis of rat dorsal root ganglion lysates (lanes 1 and 4), rat lung membranes (lanes 2 and 5) and rat heart membranes (lanes 3 and 6):
    Western blot analysis of rat dorsal root ganglion lysates (lanes 1 and 4), rat lung membranes (lanes 2 and 5) and rat heart membranes (lanes 3 and 6):
    1-3. Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (1:200).
    4-6. Anti-CRLR/CALCRL (extracellular) Antibody, preincubated with CRLR/CALCRL (extracellular) Blocking Peptide (#BLP-CR060).
  • Western blot analysis of mouse heart lysates (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    Western blot analysis of mouse heart lysates (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1, 2. Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (1:200).
    3, 4. Anti-CRLR/CALCRL (extracellular) Antibody, preincubated with CRLR/CALCRL (extracellular) Blocking Peptide (#BLP-CR060).
  • Western blot analysis of human THP-1 monocytic leukemia cell line lysate:
    Western blot analysis of human THP-1 monocytic leukemia cell line lysate:
    1. Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (1:200).
    2. Anti-CRLR/CALCRL (extracellular) Antibody, preincubated with CRLR/CALCRL (extracellular) Blocking Peptide (#BLP-CR060).
Immunohistochemistry
  • Expression of Calcitonin Receptor-Like Receptor in rat brain stem
    Expression of Calcitonin Receptor-Like Receptor in rat brain stem
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A.  CRLR staining (green) in the Raphe Magnus region is detected in neurons (arrows). B. Pre-incubation of the antibody with CRLR/CALCRL (extracellular) Blocking Peptide (#BLP-CR060), suppresses staining. Cell nuclei are stained with DAPI (blue).
  • Expression of Calcitonin Receptor-Like Receptor in mouse brain stem
    Expression of Calcitonin Receptor-Like Receptor in mouse brain stem
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CRLR/CALCRL (extracellular) (extracellular) Antibody (#ACR-060), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. CRLR staining (green) in the Raphe Magnus region is detected in (green) in neurons (arrows). B. Pre-incubation of the antibody with CRLR/CALCRL (extracellular) Blocking Peptide (#BLP-CR060), suppresses staining. Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of CRLR in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of CRLR in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (2.5 µg) + goat-anti-rabbit-FITC.
References
  1. Singh, Y. et al. (2017) CNS Neurosci. Ther. 23, 457.
  2. Conner, A.C. et al. (2002) Biochem. Soc. Trans. 30, 451.
  3. Holland, P.R. and Goadsby, P.J. (2018) Neurotherapeutics 15, 304.
Scientific background

Calcitonin Receptor-Like Receptor 1 (CRLR or CGRPR) is a G protein-coupled receptor that binds the peptide hormone calcitonin and is involved in maintenance of calcium homeostasis, particularly with respect to bone formation and metabolism1.

When engaged with its substrate, calcitonin gene related peptide (CGRP), CGRPR activates cAMP-dependent pkA and PI3 kinase, which eventually leads to complex inhibitory as well as facilitator actions on Neuronal Nicotinic Acetylcholine Receptor1.

CGRP receptor is a heterodimer composed of two proteins: a 7‐transmembrane domain type 2 G-protein coupled receptor called calcitonin-receptor-like receptor (CRLR), and receptor-activity-modifying protein 1 (RAMP1), a single-membrane-pass protein. RAMP1 serves as a transporter for CRLR to the cell surface and its absence leads to failure of binding with CRLR2.

CGRP and CGRPR are ubiquitously expressed and their interaction is related to various diseases and health states such as migraines3 and Alzheimer's disease1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

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Last update: 07/09/2020

Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and indirect live cell flow cytometry. It has been designed to recognize CRLR from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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