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Peptide CEVSVTPKTVTPAS, corresponding to amino acid residues 504-517 of rat CRMP-2 (Accession P47942). Intracellular.
Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain membrane (lanes 2 and 6), human CCF-STTGI (lanes 3 and 7) and rat PC-12 (lanes 4 and 8) cell line lysates:1-3. Anti-CRMP-2 Antibody (#AIP-029), (1:200).
4. Anti-CRMP-2 Antibody (1:500).
5-8. Anti-CRMP-2 Antibody, preincubated with the control peptide antigen.
Collapsin response mediator protein 2 (CRMP-2, DRP‐2), is a kinesin-binding protein and a member of the CRMP family, a novel family of microtubule-associated proteins (MAPs). This family consists of 5 highly conserved phosphorylated proteins: CRMP1-CRMP5. CRMP-2 was originally identified in the regulation of microtubule dimerization. CRMP-2 is the first member of the CRMP family identified in primary neuronal cells1,2.
Physiological functions of CRMP-2 protein include regulation of cell surface receptor endocytosis, kinesin-dependent axonal transport, growth cone collapse, polarity and differentiation of developing neurons and neurite outgrowth and the regulation of microtubule dynamics2.
Studies suggest that CRMP-2 can modulate neuronal viability through the PI3K/mTOR/S6K pathway. In addition, CRMP-2 expression has been detected in non-neuronal cells such as leukocytes, fibroblasts and neuroblastoma1.
Phosphorylation of CRMP-2 on specific Thr and Ser residues inactivates its activity, leading to inhibition of axonal growth and neuronal polarity. Knockdown of CRMP‐2 shortens the total dendritic length and decreases dendritic branching3.
Species reactivity key:
Anti-CRMP-2 Antibody (#AIP-029) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize DPYSL2 from rat, mouse, and human samples.