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Anti-CRMP2 Antibody

CRMP-2, Collapsin response mediator protein 2, Dihydropyrimidinase-related protein 2, DHPRP2, DRP-2, Dpysl2, Turned on after division 64 kDa protein, TOAD-64, Ulip2, Unc-33-like phosphoprotein 2
Cat #: AIP-029
Alternative Name CRMP-2, Collapsin response mediator protein 2, Dihydropyrimidinase-related protein 2, DHPRP2, DRP-2, Dpysl2, Turned on after division 64 kDa protein, TOAD-64, Ulip2, Unc-33-like phosphoprotein 2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CEVSVTPKTVTPAS, corresponding to amino acid residues 504-517 of rat CRMP2 (Accession P47942). Intracellular.
Accession (Uniprot) Number P47942
Gene ID 25416
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain membrane (lanes 2 and 6), human CCF-STTGI (lanes 3 and 7) and rat PC-12 (lanes 4 and 8) cell line lysates:
    Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain membrane (lanes 2 and 6), human CCF-STTGI (lanes 3 and 7) and rat PC-12 (lanes 4 and 8) cell line lysates:
    1-3. Anti-CRMP2 Antibody (#AIP-029), (1:200).
       4. Anti-CRMP2 Antibody (1:500).
    5-8. Anti-CRMP2 Antibody, preincubated with CRMP2 Blocking Peptide (#BLP-IP029).
References
  1. He, J. and Zhu, J. (2018) Med. Sci. Monit. 24, 4982.
  2. Mokhtar, S.H. et al. (2018) Neural Regen. Res. 13, 1066.
  3. Tan, M. et al. (2013) J. Neurochem. 125, 685.
Scientific background

Collapsin response mediator protein 2 (CRMP-2, DRP‐2), is a kinesin-binding protein and a member of the CRMP family, a novel family of microtubule-associated proteins (MAPs). This family consists of 5 highly conserved phosphorylated proteins: CRMP1-CRMP5. CRMP-2 was originally identified in the regulation of microtubule dimerization. CRMP-2 is the first member of the CRMP family identified in primary neuronal cells1,2.

Physiological functions of CRMP-2 protein include regulation of cell surface receptor endocytosis, kinesin-dependent axonal transport, growth cone collapse, polarity and differentiation of developing neurons and neurite outgrowth and the regulation of microtubule dynamics2.

Studies suggest that CRMP-2 can modulate neuronal viability through the PI3K/mTOR/S6K pathway. In addition, CRMP-2 expression has been detected in non-neuronal cells such as leukocytes, fibroblasts and neuroblastoma1.

Phosphorylation of CRMP-2 on specific Thr and Ser residues inactivates its activity, leading to inhibition of axonal growth and neuronal polarity. Knockdown of CRMP‐2 shortens the total dendritic length and decreases dendritic branching3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-CRMP2 Antibody (#AIP-029) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize CRMP-2 from rat, mouse, and human samples.

For research purposes only, not for human use
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