Anti-CX3CL1 (extracellular) Antibody

Fractalkine, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1, FKN, NTT, SCYD1
  • New
Cat #: ACR-057
Alternative Name Fractalkine, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1, FKN, NTT, SCYD1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide CGKRAIILETRQHR, corresponding to amino acid residues 58-71 of human CX3CL1 (Accession P78423). Extracellular, N-terminus.
    • Anti-CX3CL1 (extracellular) Antibody
    Accession (Uniprot) Number P78423
    Gene ID 6376
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat – identical; mouse – 12/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*, lci*
    Western blot
    • Anti-CX3CL1 (extracellular) Antibody
      Western blot analysis of rat heart membrane (lanes 1 and 5), human pancreatic carcinoma PANC-1 lysate (lanes 2 and 6), mouse heart membrane (lanes 3 and 7) and rat brain synaptosomal fraction (lanes 4 and 8):
      1-4. Anti-CX3CL1 (extracellular) Antibody (#ACR-057), (1:400).
      5-8. Anti-CX3CL1 (extracellular) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-CX3CL1 (extracellular) Antibody
      Expression of CX3CL1 in rat frontal cortex
      Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-CX3CL1 (extracellular) Antibody (#ACR-057), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. CX3CL1 staining (green) in the deep layer of the frontal cortex is detected in cortical neurons (vertical arrows) and their dendrites (horizontal arrows). B. CX3CL1 immunoreactivity (green) in the upper layer of the frontal cortex is observed in cortical neurons (vertical arrows). Cell nuclei are stained with DAPI (blue).
    References
    1. Chen, P. et al. (2016) Biomed. Res. Int. 2016, 8090918.
    2. Watson, A.E.S. et al. (2019) Neurosci. Lett. doi: 10.1016/j.neulet.2019.134533.
    3. Luo, P. et al. (2019) Brain Res. Bull. 146, 12.
    4. Fan, Q. et al. (2019) J. Exp. Med. 216, 1891.
    Scientific background

    CX3C chemokine ligand 1 (CX3CL1), also known as fractalkine (FKN), is a chemokine belonging to the CX3C family. CX3CL1 is secreted by neurons and plays an important role in modulating glial activation in the central nervous system after binding to its sole receptor CX3CR1 which mainly is expressed on microglia. The CX3CL1/CX3CR1 interaction is an important bridge connecting neuron and microglia1.

    CX3CL1 is a large cytokine protein with an extended mucin-like stalk and a chemokine domain at the N-terminus. It is the only member of CX3C family which belongs to the large family of small secreted chemotactic cytokines. CX3CL1 is highly and constitutively expressed in hippocampal and cortical neurons. It exists in both secreted and membrane-bound form. While the secreted form contains only the chemokine domain located in the N-terminus, the membrane-bound form contains the chemokine domain, a mucin-like stalk, a transmembrane domain and an intracellular C-terminal domain. The membrane-tethered mucin stalk acts as a cell adhesion molecule adhering to microglia during an inflammatory reaction, it can be cleaved to produce a soluble glycoprotein by metalloproteinases ADAM 10 and ADAM 17 or lysosomal cysteine protease and cathepsin S (CatS), The soluble form has been proposed to act as a chemoattractant and can serve as a signaling molecule mediating neural/microglial interactions via its receptor CX3CR1, mainly expressed on microglia and partly on astrocyte as well as on neurons in the CNS. The CX3CL1/CX3CR1 interaction on microglia is likely to alter the microglial state to a more neuroprotective one.  Notably, transmembrane and soluble CX3CL1 elicit different cytokine responses in immune cells1-3.

    CX3CL1 has been associated with learning and memory as CX3CL1 is upregulated in the rat hippocampus during memory-associated synaptic plasticity. It is considered as a potent neuromodulator of the evoked excitatory synaptic transmission and plays a major role in synaptic plasticity and neuroprotection1,4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 22/03/2020

    Anti-CX3CL1 (extracellular) Antibody (#ACR-057) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize CX3CL1 from human, mouse and rat samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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