Anti-xCT/SLC7A11 (extracellular) Antibody

Cystine/glutamate transporter, Amino acid transport system xc-, Solute carrier family 7 member 11, Calcium channel blocker resistance protein CCBR1
    Cat #: ANT-111
    Alternative Name Cystine/glutamate transporter, Amino acid transport system xc-, Solute carrier family 7 member 11, Calcium channel blocker resistance protein CCBR1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)THHFKDAFSGRDTS, corresponding to amino acid residues 218-231 of mouse cystine/glutamate transporter (Accession Q9WTR6). 3rd extracellular loop.
        Anti-xCT/SLC7A11 (extracellular) Antibody
    Accession (Uniprot) Number Q9WTR6
    Gene ID 26570
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat – identical; human – 11/14 amino acid residues identical.
    RRID AB_2756725.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ih*, ip*, lci*
      • Anti-xCT/SLC7A11 (extracellular) Antibody
        Western blot analysis of human brain glioblastoma cell line (U-87 MG) lysate:
        1. Anti-xCT/SLC7A11 (extracellular) Antibody (1:200).
        2. Anti-xCT/SLC7A11 (extracellular) Antibody, preincubated with the negative control antigen.
        Anti-xCT/SLC7A11 (extracellular) Antibody
        Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat stomach (lanes 3 and 6) lysates:
        1-3. Anti-xCT/SLC7A11 (extracellular) Antibody (#ANT-111), (1:800).
        4-6. Anti-xCT/SLC7A11 (extracellular) Antibody, preincubated with the negative control antigen.
    References
    • 1. Verrey, F. et al. (2004) Pflugers Arch. 447, 532.
    • 2. Liu, R. et al. (2007) Mol. Pharmacol. 72, 1637.
      • The SLC7 family is divided into two subgroups, the cationic amino acid transporters (the CAT family, SLC7A1–4) and the glycoprotein-associated amino acid transporters (the gpaAT family, SLC7A5–11) also called light chains or catalytic chains of the hetero(di)meric amino acid transporters (HAT). The gene SLC7A11 encodes the xCT protein (also known as Cystine/glutamate transporter).

        xCT, as well as other HATs, is comprised of a light chain with 12 non-glycosylated transmembrane helices and is associated with the glycosylated heavy chain 4F2hc (CD98, SLC3A2) or rBAT (SLC3A1) through a conserved disulphide bridge. The COOH-terminus of the xCT is localized intracellularly. Association of the light and heavy chains is required for surface expression of the transporter which forms the heterodimeric amino acid transport system xc−. xCT is mainly expressed macrophages, brain, retinal pigment cells, liver and kidney. xCT expression is elevated in cells requiring high glutathione synthesis, such as activated macrophages, neuronal and glial cells and other cells after glutathione depletion1.

        xCT operates in a Na+-independent and electroneutral mode, exchanging extracellular anionic cystine (with pH dependence) for glutamate with a stoichiometry of 1:1. The driving force for this exchange is generated by the cystine concentration gradient (intracellular reduction) and the high intracellular concentration of glutamate.

        xCT has been suggested as a mediator of treatment resistance in cancer patients. In tumor cells, xCT plays a crucial role in regulating intracellular levels of glutathione which has been broadly implicated in resistance to chemotherapy2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry,
    IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 20/10/2019

    Anti-xCT/SLC7A11 (extracellular) Antibody (#ANT-111) is a highly selective antibody directed against an epitope of the mouse cystine/glutamate transporter. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize xCT from human, mouse, and rat samples.

    For research purposes only, not for human use