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- Peptide (C)EGPWERPPPLDESER, corresponding to amino acid residues 160 - 174 of human DARPP32 (Accession Q9UD71). Intracellular, C-terminus.
- Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1, 2. Anti-DARPP32 Antibody (#ADR-006), (1:200).
3, 4. Anti-DARPP32 Antibody, preincubated with the negative control antigen.Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 3) and human HT-29 colorectal adenocarcinoma cell line lysate (lanes 2 and 4):1, 2. Anti-DARPP32 Antibody (#ADR-006), (1:200).
3, 4. Anti-DARPP32 Antibody, preincubated with the negative control antigen.
- Expression of DARPP32 in rat striatumImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-DARPP32 Antibody (#ADR-006), (1:200), followed by goat anti-rabbit-AlexaFluor-488. DARPP32 immunoreactivity (green) appears in striatal cells (arrows). ST=striatum, CC=corpus callosum. Cell nuclei are stained with DAPI (blue).
- 1. Avanes, A. et al. (2019) Biochem. Pharmacol. 160, 71.
- 2. Yamada, K. et al. (2016) J. Physiol. Sci. 66, 435.
- 3. Momand, J. et al. (2017) FEBS Open Bio 7, 1328.
- 4. Greengard, P. et al. (1999) Neuron 23, 435.
- 5. Belkhiri, A. et al. (2016) Oncotarget 7, 17631.
- 6. Svenningsson, P. et al. (2004) Annu. Rev. Pharmacol. Toxicol. 44, 269.
Dopamine- and cAMP-regulated neuronal phosphoprotein (DARPP32), also known as, Protein phosphatase 1 regulatory subunit 1B (PPP1R1B) is a protein regulated by the phosphorylation of cAMP1.
DARPP32 is expressed in the spiny neurons of the neostriatum and in the adrenal medulla, kidney, and parathyroid cells and it is also overexpressed in tumor cells from breast, colon, esophagus, lung and prostate tissues1,2. Its protein structure is comprised of 18% alpha helix, 23% beta strand, 30% beta turn, and 29% random coil3.
DARPP32 has been shown to play a key role in mediating the biochemical, electrophysiological, and behavioral effects of dopamine on dopaminoceptive neurons4,5. It is also involved in mediating the actions of other neurotransmitter systems such as serotonin and glutamate, in response to a variety of drugs5,6.
DARPP32 is reported to have a role in development and neurodegenerative diseases. In addition, DARPP32 plays a role in cancer tumorigenesis. Collectively, findings from published reports suggest DARPP32 as a potential biomarker of drug resistance and a therapeutic target in gastric cancer and other malignancies5.
Species reactivity key:
Anti-DARPP32 Antibody (#ADR-006) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize DARPP32 from human, rat, and mouse samples.
- Anti-D1 Dopamine Receptor Antibody (#ADR-001)
- Anti-D2 Dopamine Receptor (extracellular) Antibody (#ADR-002)
- Anti-D2 Dopamine Receptor (extracellular)-FITC Antibody (#ADR-002-F)
- Anti-D5 Dopamine Receptor (extracellular) Antibody (#ADR-005)
- Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001)
- Anti-NMDAR2A (GluN2A) (extracellular) Antibody (#AGC-002)
- Anti-NMDAR2A (GluN2A) (extracellular)-ATTO-488 Antibody (#AGC-002-AG)
- Anti-NMDAR2B (GluN2B) (extracellular) Antibody (#AGC-003)
- Anti-NMDAR2B (GluN2B) (extracellular)-ATTO-594 Antibody (#AGC-003-AR)