- Peptide (C)DAHASNSSDGLGLND, corresponding to amino acids residues 191-205 of rat DAT (Accession P23977). 2nd extracellular loop.
- Western blot analysis of rat (lanes 1 and 4) and mouse (lanes 2 and 5) brain membranes and rat pheochromocytoma PC12 (lanes 3 and 6) cell lysates:1-3. Anti-Dopamine Transporter (DAT) (extracellular) Antibody (#AMT-003), (1:200).
4-6. Anti-Dopamine Transporter (DAT) (extracellular) Antibody, preincubated with Dopamine Transporter/DAT (extracellular) Blocking Peptide (#BLP-MT003).
- Mouse coronal brain sections (1:200) (Hong, J. et al. (2015) Cell Death Dis. 6, e1832.).
- Cell surface detection of DAT by indirect flow cytometry in live intact mouse J774 macrophage cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-Dopamine Transporter (DAT) (extracellular) Antibody (#AMT-003), (2.5μg) + goat-anti-rabbit-FITC.
- Expression of DAT in rat PC12 cellsCell surface detection of DAT in live intact rat pheochromocytoma PC12 cells. A. Cells stained with Anti-Dopamine Transporter (DAT) (extracellular) Antibody (#AMT-003), (1:100), followed by goat anti-rabbit-AlexaFluor- 594 secondary antibody (red). B. Live view of the cells.
The Na+/Cl- transporter family SLC6, includes DAT, a monoamine transporter, important for regulating extracellular levels of dopamine. It does so by taking up dopamine from the synaptic cleft via the co-transport of Na+ and Cl- down their electrochemical gradients1. The removal of dopamine by DAT remains the most important means to control the extracellular lifetime of the neurotransmitter and notably, the ending of dopaminergic neurotransmission2. Transporters for serotonin, norepinephrine, GABA and glycine also belong to this family3.
These receptors have 12 transmembrane spanning domains and intracellular N- and C-termini. DAT is also subject to post translational modifications such as phosphorylation, important for its regulation1. It also possesses a large extracellular domain which undergoes N-glycosylation, important for the proper targeting of the transporter to the plasma membrane1. DAT is expressed in dopaminergic cell bodies and terminals and can therefore serve as a marker for these neurons4. DAT is also expressed in the retina, gastrointestinal tract, lung, kidney, pancreas and lymphocytes2.
DAT plays an important role in movement as well as reward, learning and memory5. Its malfunction, which leads to dopaminergic dysregulation, has been associated with ADHD, schizophrenia, as well as Parkinson’s disease2. DAT is the main target for cocaine, amphetamine and methamphetamine psychostimulants, which mainly increase locomotor activities2.
Species reactivity key:
Expression of Dopamine Transporter in mouse brain.Western blot analysis of mouse midbrain lysates using Anti-Dopamine Transporter (DAT) (extracellular) Antibody (#AMT-003) (upper panel). DAT protein levels from mice exposed to different drugs are shown. Immunohistochemical staining of mouse brain sections (lower panel). DAT staining (red) in substantia nigra is detected in dopaminergic neurons.Adapted from Hong, J. et al. (2015) Cell Death Dis. 6, e1832. with permission of Nature Publishing Group.
Anti-Dopamine Transporter (DAT) (extracellular) Antibody (#AMT-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize DAT from mouse and rat samples. The antibody is unlikely to recognize DAT from human samples.
- Anti-Dopamine Transporter (DAT) (extracellular)-FITC Antibody (#AMT-003-F). This FITC-conjugated antibody can be used to detect DAT in live cell flow cytometry.
- Anti-D1 Dopamine Receptor Antibody (#ADR-001)
- Anti-D2 Dopamine Receptor (extracellular) Antibody (#ADR-002)
- Anti-D3 Dopamine Receptor (extracellular) Antibody (#ADR-003)
- Anti-D4 Dopamine Receptor Antibody (#ADR-004)
- Anti-D5 Dopamine Receptor (extracellular) Antibody (#ADR-005)
- Anti-Noradrenaline Transporter (NET) (extracellular) Antibody (#AMT-002)
- Anti-Serotonin Transporter (SERT) (extracellular) Antibody (#AMT-004)