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Anti-Endothelin Receptor B (extracellular) Antibody

EDNRB, ET-B, ETB, ETRB, Endothelin B receptor, ETBR

Cat #: AER-005
Alternative Name EDNRB, ET-B, ETB, ETRB, Endothelin B receptor, ETBR
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)KTSWTRGSNSSLMR, corresponding to amino acid residues 52 - 65 of rat EDNRB (Accession P21451). Extracellular, N-terminus.
Accession (Uniprot) Number P21451
Gene ID 50672
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Specificity The antibody is not recommended for recognizing Endothelin Receptor B in human samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ifc, lci, wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and rat dorsal root ganglion lysate (lanes 3 and 6):
    Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and rat dorsal root ganglion lysate (lanes 3 and 6):
    1-3. Anti-Endothelin Receptor B (extracellular) Antibody (#AER-005), (1:200).
    4-6. Anti-Endothelin Receptor B (extracellular) Antibody, preincubated with Endothelin Receptor B (extracellular) Blocking Peptide (BLP-ER005).
  • Western blot analysis of mouse J774 macrophage cell line lysate (lanes 1 and 3) and  rat aortic endothelial cells lysate (lanes 2 and 4):
    Western blot analysis of mouse J774 macrophage cell line lysate (lanes 1 and 3) and  rat aortic endothelial cells lysate (lanes 2 and 4):
    1-2. Anti-Endothelin Receptor B (extracellular) Antibody (#AER-005), (1:200).
    3-4. Anti-Endothelin Receptor B (extracellular) Antibody, preincubated with Endothelin Receptor B (extracellular) Blocking Peptide (BLP-ER005).
Indirect flow cytometry
  • Cell surface detection of Endothelin  Receptor B  by indirect flow cytometry in live intact mouse  J774 macrophage cell line:
    Cell surface detection of Endothelin  Receptor B  by indirect flow cytometry in live intact mouse  J774 macrophage cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-Endothelin Receptor B (extracellular) Antibody (#AER-005), (2.5μg) + goat-anti-rabbit-FITC.
References
  1. Davenport, A.P. (2002) Pharmacol. Rev54, 219.
  2. Boivin, B. et al. (2003) J. Biol. Chem. 278, 29153.
  3. Grant, K. et al. (2003) Br. J. Cancer88, 163.
Scientific background

Endothelins (ET-1, 2, and 3) are considered to be very powerful vasoconstrictive substances. In humans, endothelins mediate their actions via two specific G-protein coupled receptors, ETAR and ETBR. Both ETAR and ETBR are present in the heart and in human myocardium at similar levels.1,2

The endothelin receptors differ in their ligand specificity. While ETAR has varying affinities for the endothelin isoforms (ET-1 > ET-2 > ET-3), ETBR shows no selective affinity.2,3 

Subsequent studies have demonstrated the presence of endothelins in vascular, as well as in non-vascular, cells and tissues, having multiple biological activities.

Currently, there is increasing evidence that ET-1 may modulate mitogenesis, apoptosis, angiogenesis tumor invasion and the development of metastases.3

Until recently, it was thought that all cellular activities of the endothelins were mediated through their interactions with their cell surface receptors. However, a recent study demonstrated that cardiac nuclei also possess both ETAR and ETBR subtypes, which are functional and coupled to signaling mechanisms within the nuclear membrane.2

Hypermethylation of the ETBR correlated with transcriptional down-regulation and reduced expression of ETB receptor was observed in several prostate, bladder and colon cancer cell lines.3

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 30/09/2021

Anti-Endothelin Receptor B (extracellular) Antibody (#AER-005) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot and flow cytometry applications. It has been designed to recognize Endothelin Receptor B from rat and mouse samples. The antibody is not recommended for human samples.

For research purposes only, not for human use
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