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Anti-EphA1 (extracellular)-FITC Antibody

Ephrin type-A receptor 1, Eph receptor A1, EPH tyrosine kinase 1, Tyrosine-protein kinase receptor EPH, EPHT1

Cat #: AER-011-F
Alternative Name Ephrin type-A receptor 1, Eph receptor A1, EPH tyrosine kinase 1, Tyrosine-protein kinase receptor EPH, EPHT1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KKEPRQLELTWAGSR, corresponding to amino acid residues 457-471 of human EphA1 (Accession P21709). Extracellular, N-terminus.
Accession (Uniprot) Number P21709
Gene ID 2041
Homology Mouse, rat – identical
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Label Fluorescein isothiocyanate (FITC)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #AER-011), and direct flow cytometry (labeled antibody).
Applications: fc, lci
Direct flow cytometry
  • Cell surface detection of EphA1 by direct flow cytometry in live intact mouse J774 macrophage cells:
    Cell surface detection of EphA1 by direct flow cytometry in live intact mouse J774 macrophage cells:
    ___ Cells.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + Anti-EphA1 (extracellular)-FITC Antibody (#AER-011-F), (2.5µg) .
  • Cell surface detection of EphA1 by direct flow cytometry in live intact human Jurkat T-cell leukemia cells:
    Cell surface detection of EphA1 by direct flow cytometry in live intact human Jurkat T-cell leukemia cells:
    ___ Cells.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + EphA1 (extracellular)-FITC Antibody (#AER-011-F), (5µg).
References
  1. Chen, Y. et al. (2012) Cell. Signal. 24, 606.
  2. Pasquale, E.B. (2008) Cell 133, 38.
  3. Pasquale, E.B. (2005) Mol. Cell Biol. 6, 462.
  4. Du, J. et al. (2007) Curr. Pharm. Des. 13, 2507.
  5. Wu, J. and Luo, H. (2005) Curr. Opin. Hematol. 12, 292.
  6. Ireton, R.C. and Chen, J. (2005) Curr. Cancer Drug Targets 5, 149.
  7. Noren, N.K. and Pasquale, E.B. (2007) Cancer Res. 67, 3994.
Scientific background

Eph receptors are the largest family of receptor tyrosine kinases (RTKs). EphA receptors (EphA1-10) bind ephrinA ligands which are GPI-linked proteins and EphB receptors (EphB1-6) bind ephrinB ligands which are membrane protein with one transmembrane domain1,2. Within each subfamily, interactions between receptor and ligand are promiscuous. In addition, Eph receptors can also bind ephrins from the other class2. Forward and reverse signaling through Eph receptors is a unique characteristic to this RTK since ephrins are physically linked to the plasma membrane3.

Structurally, Eph receptors contain an extracellular ligand-binding domain, a transmembrane domain and an intracellular C-terminal domain responsible for intracellular signaling1. Forward Eph receptor signaling involves autophosphorylation of the receptor via a tyrosine kinase domain, as well as phosphorylation of other proteins. Known effectors of the forward signaling include Src kinase and Ras/Rho GTPases2. Much less is known about the reverse signaling mediated by Eph receptors. Besides from acting independently, Eph receptors can also signal in concert with other receptors. For example, Eph receptors cooperate with FGF receptor, NMDA ligand-gated ion channel and chemokine G-protein coupled receptor2.

Biological activities attributed to the Eph receptor-ephrin signaling module include establishing neuronal connections, mediating neuronal plasticity and repair following neuronal injury2,4. Eph receptors may also have a role in the immune system5.

Eph receptors are expressed in the developing nervous system, and in the adult brain. It is also detected in the pancreas, intestine, bone and lymphocytes. In cancer cells, Eph receptors and ephrins are overexpressed2,6,7. They are also implicated in neurodegenerative disorders like Alzheimer’s disease1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 03/06/2021

Anti-EphA1 (extracellular) Antibody (#AER-011) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, live cell imaging, and indirect live cell flow cytometry applications. It has been designed to recognize EphA1 from rat, mouse, and human samples.

Anti-EphA1 (extracellular)-FITC Antibody (#AER-011-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

For research purposes only, not for human use
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