Anti-EphA6 (extracellular) Antibody

Ephrin type-A receptor 6, EPH receptor A6, EPH homology kinase 2, EHK-2
  • New
Cat #: AER-016
Alternative Name Ephrin type-A receptor 6, EPH receptor A6, EPH homology kinase 2, EHK-2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    • Peptide (C)KEHEQLTYSSTRSK, corresponding to amino acid residues 482-495 of rat EphA6 (Accession P54758). Extracellular, N-terminus.
    • Anti-EphA6 (extracellular) Antibody
    Accession (Uniprot) Number P54758
    Gene ID 29202
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human – identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, ih, wb
    May also work in: ic*, ip*, lci*
    Western blot
    • Anti-EphA6 (extracellular) Antibody
      Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
      1, 2. Anti-EphA6 (extracellular) Antibody (#AER-016), (1:200).
      3, 4. Anti-EphA6 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-EphA6 (extracellular) Antibody
      Western blot analysis of human LNCaP prostate adenocarcinoma cell line lysate:
      1. Anti-EphA6 (extracellular) Antibody (#AER-016), (1:400).
      2. Anti-EphA6 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-EphA6 (extracellular) Antibody
      Expression of EphA6 in rat parietal cortex
      Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-EphA6 (extracellular) Antibody (#AER-016), (1:200), followed by goat anti-rabbit-AlexaFluor-488. EphA6 immunoreactivity (green) appears in pyramidal cells (arrows). Cell nuclei are stained with DAPI (blue).
    Indirect flow cytometry
    • Anti-EphA6 (extracellular) Antibody
      Cell surface detection of EphA6 in live intact human THP-1 monocytic leukemia cells:
      ___ Cells.
      ___ Cells + goat-anti-rabbit-FITC.
      ___ Cells + Anti-EphA6 (extracellular) Antibody (#AER-016), (2.5 µg) + goat-anti-rabbit-FITC.
    1. Zhou, D. et al. (2018) Oncol. Lett. 15, 5257.
    2. Das, G. et al. (2016) Cell Biosci. 6, 48.
    3. Teng, S. et al. (2019) Brain Inj. 33, 1385.
    4. Darling, T.K. et al. (2019) Front. Immunol. 10, 1473.
    Scientific background

    EphA6 is an erythropoietin-producing hepatocellular (Eph) receptor that belongs to the tyrosine kinase receptor family. The Eph receptor family consists of 9 EphA receptors and 5 EphB receptors, which serve key roles in normal physiology, including cell migration, cell adhesion, cell proliferation and neuronal synaptic plasticity. These activities depend on the binding of their Eph receptor-interacting (ephrin) proteins, of which include 8 members. Compelling data has demonstrated that Eph receptors and ephrins affect tumor growth, invasiveness, angiogenesis and metastasis1. They are also involved in brain development, learning and memory2.

    EphA6 signaling pathways are inhibitory for developing axons and blocking these pathways enhances regeneration following spinal cord and brain injury; rendering EphA6 an appealing drug target3.

    Eph receptors contain an extracellular structure consisting of an ephrin binding domain, the juxtamembrane region connects the extracellular portion of the receptor to the intracellular kinase domain. Two Tyrosine residues on the juxtamembrane region mediate autophosphorylation and receptor dimerization mediates the formation of heterocomplexes that are required for signaling4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 11/02/2020

    Anti-EphA6 (extracellular) Antibody (#AER-016) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize EphA6 from rat, mouse, and human samples.

    For research purposes only, not for human use
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