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This product is freeze dried. All water molecules have been removed.
This antibody is shipped with its antigen FREE of charge!
This product is "conjugated" to a fluorescent dye.
This antibody has undergone quality control and has been approved by our professional team.
This lyophilized product is shipped at room temperature. Please see its certificate of analysis for further storage instructions.
___ Cells + Rabbit IgG isotype control-FITC.
___ Cells + Anti-Excitatory Amino Acid Transporter 1 (extracellular)-FITC antibody (#AGC-021-F), (5 µg).
Glutamate transporters play important roles in the termination of excitatory neurotransmission and in providing cells with glutamate for metabolic purposes.
SLC1A3 (EAAT1) belongs to The SLC1 protein family which contains a range of human and prokaryotic glutamate and aspartate transporters. EAAT1 transports the acidic amino acids glutamate and aspartate against a concentration gradient generating a net movement of substrate and charge across the membrane. Glutamate transport by EAAT1 is coupled to the co-transport of three Na+ ions and one H+ ion, and the counter transport of one K+ ion1.
EAAT1 is mainly expressed in astrocytes but can also be found in oligodendrocytes. EAAT1, together with EAAT2, are responsible for the bulk of glutamate uptake in the brain. They have several important roles, including the termination of the synaptic effects of glutamate, prevention of potentially toxic accumulation of extracellular glutamate, supply of glutamate to the astrocyte for synthesis of glutamine, which is involved in ammonia detoxification and in the glutamine-glutamate cycle and signaling of the metabolic needs of nearby neurons and activation of glycolysis in astrocytes2.
EAAT1 has been implicated in the pathogenesis of episodic ataxia, a rare neurological disorder characterized by neonatal muscle stiffness and an exaggerated startle reflex induced by noise or touch3.
Species reactivity key:
Anti-Excitatory Amino Acid Transporter 1 (extracellular) (#AGC-021) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize EAAT1 from rat, human and mouse samples.
Alomone Labs is pleased to offer a new version of this antibody that is directly conjugated to fluorescein isothiocyanate (FITC). Anti-Excitatory Amino Acid Transporter 1 (extracellular)-FITC antibody (#AGC-021-F) can be used in immunofluorescent applications such as live cell direct flow cytometry.
- Anti-Excitatory Amino Acid Transporter 1 (extracellular) antibody (#AGC-021), (for western blot analysis).
- Anti-Excitatory Amino Acid Transporter 1 (extracellular) (#AGC-021)
- Anti-Excitatory Amino Acid Transporter 2 (extracellular) (#AGC-022)
- Anti-Excitatory Amino Acid Transporter 5 (extracellular) (#AGC-025)
- Anti-Excitatory Amino Acid Transporter 4 (extracellular) (#AGC-024)
- Anti-Excitatory Amino Acid Transporter 3 (#AGC-023)
- Anti-Vesicular Glutamate Transporter 1 (#AGC-035)
- Anti-Vesicular Glutamate Transporter 2 (#AGC-036)