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Anti-Frizzled-1 (FZD1) (extracellular) Antibody

Frizzled class receptor 1, Fz-1

Cat #: AFR-041
Alternative Name Frizzled class receptor 1, Fz-1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)PEFWTSNPQHGGG, corresponding to amino acid residues 238-250 of rat Frizzled-1 (Accession Q08463). Extracellular, N-terminus.
Accession (Uniprot) Number Q08463
Gene ID 58868
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
RRID AB_2756593.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, lci, wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) kidney lysates:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) kidney lysates:
    1,2. Anti-Frizzled-1 (FZD1) (extracellular) Antibody (#AFR-041), (1:200).
    3,4. Anti-Frizzled-1 (FZD1) (extracellular) Antibody, preincubated with Frizzled-1/FZD1 (extracellular) Blocking Peptide (#BLP-FR041).
Indirect flow cytometry
  • Cell surface detection of Frizzled-1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of Frizzled-1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC
    ___ Cells +  Anti-Frizzled-1 (FZD1) (extracellular) Antibody (#AFR-041), (2.5μg) + goat-anti-rabbit-FITC
References
  1. Alexander, S.P. et al. (2013) Br. J. Pharmacol. 170, 1459.
  2. Wang, S. et al. (2013) Biotech. Lett. 35, 1199.
  3. Zhang, H. et al. (2012) Cancer Lett. 323, 106.
Scientific background

Frizzled class receptor 1 (also known as FZD1) belongs to the frizzled subfamily of G protein-coupled receptors. Currently, the frizzled family of GPCRs contains 10 frizzled proteins (FZD1-10) and Smoothened (SMO).

Similar to other GPCRs, FZD1 consists of a single polypeptide with an extracellular N-terminus, an intracellular C-terminus, seven hydrophobic transmembrane domains (TM1-TM7) linked by three extracellular loops (ECL1-ECL3) and three intracellular loops (ICL1-ICL3). FZD1 is encoded by the firzzled-1 gene which is mainly expressed in the adult heart, placenta, lung, kidney, pancreas, prostate and ovary.

FZD1 is activated by WNTs, which are cysteine-rich lipo-glycoproteins with fundamental functions in ontogeny and tissue homeostasis. Signaling through the WNT/FZD complex can lead to the activation of pertussis toxin-sensitive heterotrimeric G proteins, the elevation of intracellular calcium activation of cGMP-specific PDE6 and elevation of cAMP as well as RAC-1, JNK, Rho and Rho kinase signaling1.

The relation between the FZD1 pathway and various pathologies has been researched extensively. Reduced levels of FZD1 can be found in the spinal cords of ALS transgenic mice, possibly due to the increase of FZD1 protein degradation or the inhibition of FZD1 protein expression in the spinal cord in the disease process of ALS transgenic mice2. The FZD1 signaling pathway has also been implicated in inflammatory bowel disease. Patients with ulcerative colitis exhibit reduced expression of FZD1. FZD1 is overexpressed in the multi drug resistant cancer cell subline MCF-7/ADM and seems to mediate multidrug resistance by regulating the Wnt/β-catenin pathway3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Anti-Frizzled-1 (FZD1) (extracellular) Antibody (#AFR-041) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize FZD1 from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

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