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Anti-Frizzled-2 (FZD2) (extracellular) Antibody

Frizzled class receptor 2, Fz-2

Cat #: AFR-022
Alternative Name Frizzled class receptor 2, Fz-2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, r
May also work in: m*
Immunogen
  • Peptide (C)GAPPRYATLEHPFH, corresponding to amino acid residues 192-205 of rat Frizzled-2 (Accession Q08464). Extracellular, N-terminus.
Accession (Uniprot) Number Q08464
Gene ID 64512
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human - identical; mouse - 13/14 amino acid residues identical.
RRID AB_2756591.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, lci, wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of rat heart lysate:
    Western blot analysis of rat heart lysate:
    1. Anti-Frizzled-2 (FZD2) (extracellular) Antibody (#AFR-022), (1:200).
    2. Anti-Frizzled-2 (FZD2) (extracellular) Antibody, preincubated with Frizzled-2/FZD2 (extracellular) Blocking Peptide (#BLP-FR022).
  • Western blot analysis of human MDA-231 breast carcinoma cell line lysate (lanes 1 and 3) and human THP-1 acute monocytic leukemia cell line lysate (lanes 2 and 4):
    Western blot analysis of human MDA-231 breast carcinoma cell line lysate (lanes 1 and 3) and human THP-1 acute monocytic leukemia cell line lysate (lanes 2 and 4):
    1,2. Anti-Frizzled-2 (FZD2) (extracellular) Antibody (#AFR-022), (1:200).
    3,4. Anti-Frizzled-2 (FZD2) (extracellular) Antibody, preincubated with Frizzled-2/FZD2 (extracellular) Blocking Peptide (#BLP-FR022).
Indirect flow cytometry
  • Cell surface detection of FZD2 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of FZD2 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-Frizzled-2 (FZD2) (extracellular) Antibody (#AFR-022), (2.5μg) + goat-anti-rabbit-FITC.
References
  1. Schulte, G. (2010) Pharmacol. Rev. 62, 632.
  2. Foord, S.M. et al. (2005) Pharmacol. Rev. 57, 279.
  3. Dijksterhuis, J.P. et al. (2014) Br. J. Pharmacol. 171, 1195.
Scientific background

Frizzled receptors (FZD) belong to the superfamily of G-protein coupled receptors (GPCR). They are classified to the frizzled subfamily for which 11 members have been identified to date; FZD1-10 and Smoothened1. They were first identified in Drosophila melanogaster.

Like all GPCRs, Frizzled receptors have seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail. However, these receptors lack many features of the conventional class A, B or C GPCRs2.

Activation of these receptors by WNT, leads to numerous complex signaling pathways which are generally termed β-catenin-dependent and β-catenin-independent. It is noteworthy to mention that the β-catenin-dependent pathway involves the interaction of FZD with LRP6 or LRP5 co-receptors3.

Frizzled receptors are ubiquitously expressed and play a crucial role in embryo development. They are also significantly involved in proliferation and differentiation. Indeed, dysregulation of FZD leads to various forms of cancer1. Scientific evidence also shows their involvement in axonal remodeling and neurotransmitter release1.

They have been associated to Alzheimer’s and Parkinson’s diseases, depression, anxiety, hypoxia and ischemia. It is thus not surprising that they have become pharmacological targets in a number of diseases and disorders.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 01/09/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Frizzled-2. Anti-Frizzled-2 (FZD2) (extracellular) Antibody (#AFR-022) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize FZD2 from rat, mouse and human samples.

For research purposes only, not for human use
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