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Anti-Frizzled-3 (FZD3) Antibody

Frizzled Class Receptor 3, Fz-3

Cat #: AFR-023
Alternative Name Frizzled Class Receptor 3, Fz-3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KVSSYHGSLHRSRDGR, corresponding to amino acid residues 581 - 596 of mouse Frizzled-3 (Accession Q61086). Intracellular, C-terminus.
Accession (Uniprot) Number Q61086
Gene ID 14365
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human, rat – identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1-2. Anti-Frizzled-3 (FZD3) Antibody (#AFR-023), (1:200).
    3-4. Anti-Frizzled-3 (FZD3) Antibody, preincubated with Frizzled-3 (FZD3) Blocking Peptide (BLP-FR023).
  • Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
    Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
    1. Anti-Frizzled-3 (FZD3) Antibody (#AFR-023), (1:200).
    2. Anti-Frizzled-3 (FZD3) Antibody, preincubated with Frizzled-3 (FZD3) Blocking Peptide (BLP-FR023).
Immunohistochemistry
  • Expression of Frizzled-3 in mouse striatum.
    Expression of Frizzled-3 in mouse striatum.
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Frizzled-3 (FZD3) Antibody (#AFR-023), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Frizzled-3 immunoreactivity (green) appears in neuronal profiles (arrows). B. Pre-incubation of the antibody with Frizzled-3 (FZD3) Blocking Peptide (BLP-FR023), suppressed staining. Cell nuclei are stained with DAPI (blue).
References
  1. Wang (2016), Genet. Mol. Res. 15 (1): gmr.15017152.
  2. Yanfeng. et al. (2006), Biol Chem. , 281, 11603.
  3. Chang. et al. (2014), J Dermatol Sci, 75, 100.
  4. Hung. et al. (2001), J Invest Dermatol, 116, 940.
  5. Kishimoto. et al. (2008), Behav Brain Funct., 4, 37.
Scientific background

Frizzled 3, encoded by FZD3 gene, is a receptor in the Wnt/β-catenin pathway, a conserved signaling pathway that regulates gene expression and controls diverse developmental processes 1. It belongs to the Frizzled protein family that contains multiple hydrophobic domains predicted to form seven transmembrane helices, similar to G protein coupled receptors. Conserved sequences are found in the Frizzled proteins extracellular cysteine-rich domain (CRD), the putative transmembrane domains, parts of the intracellular loops and a juxtamembrane motif, KTXXXW, found in the membrane-proximal C-terminal region of all Frizzleds. The C-terminal tails, however, are highly divergent 2.  

Frizzled-3 is constitutively expressed in murine melanocyte precursors, melanocytes and human melanocytes 3, it is also expressed in the epidermis of the developing embryo 4 and its expression levels are changing between different parts of the ovary throughout the process of embryogenesis 1. Because of its crucial role in regulating proper embryogenesis and its role in the development of the central nervous system, including synaptogenesis and structural plasticity, mutations in FZD3  gene were shown to have a significant association with the development of schizophrenia and other psychotic disorders 5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-Frizzled-3 (FZD3) Antibody (#AFR-023) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Frizzled-3 from mouse, rat and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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