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Anti-GABARAP Antibody

GABA receptor associated protein, GABARAPL

Cat #: AIP-016
Alternative Name GABA receptor associated protein, GABARAPL
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KFVYKEEHPFEKRR, corresponding to amino acid residues 2-15 of rat GABARAP (Accession P60517). Intracellular, N-terminus.
Accession (Uniprot) Number P60517
Gene ID 58974
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
RRID AB_2756655.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Due to high homology with GABA receptor associated protein-like proteins the antibody will recognize:
GABA receptor associated protein-like 1 (11/14 amino acid residues identical), (Accession Q0VGK0).
GABA receptor associated protein-like 3 (11/14 amino acid residues identical), (Accession Q9BY60).
May recognize GABA receptor associated protein-like 2 (8/14 amino acid residues identical), (Accession P60522).
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of mouse brain (lanes 1 and 3), rat brain (lanes 2 and 4) and human glioblastoma (SH-SY5Y) cell line (lanes 3 and 6) lysates:
    Western blot analysis of mouse brain (lanes 1 and 3), rat brain (lanes 2 and 4) and human glioblastoma (SH-SY5Y) cell line (lanes 3 and 6) lysates:
    1-3. Anti-GABARAP Antibody (#AIP-016), (1:800).
    4-6. Anti-GABARAP Antibody, preincubated with GABARAP Blocking Peptide (#BLP-IP016).
References
  1. Wang, H. et al. (1999) Nature 397, 69.
  2. Chen, Z. and Olsen, R.W. (2007) J. Neurochem. 100, 279.
Scientific background

GABA(A) receptors are ligand-gated chloride channels that mediate inhibitory neurotransmission. GABA associated protein (GABARAP) family interacts specifically with the γ1, γ2S and γ2L subunits of the GABA(A) receptor. GABARAP is expressed in a variety of tissues including heart, brain, placenta, liver and others. The genetic sequence encoding GABARAP is similar to microtubule-associated proteins 1A and 1B1.

GABARAP has an asymmetric structure with a basic N-terminal region and an acidic C terminus. GABARAP has a compact fold consisting of four or five-stranded β-sheets with two α-helices on each side. The outer strands of the β-sheets are aligned anti-parallel to the inner strands and helices α3 and α4 are located on one side of the sheet. The presence of α1 and α2 is a unique feature of GABARAP and forms a hydrophilic patch suitable for protein-protein interface. GABARAP exists in a monomeric conformation at low protein concentration and a head-to-tail oligomeric conformation in high salt conditions.

GABARAP is involved in intracellular trafficking and is an integral part of the intra-Golgi transport machinery. Thus it is detected in cellular compartments such as the Golgi apparatus and endoplasmic reticulum. GABARAP affects GABA(A) receptors’ cell surface expression by increasing their intracellular trafficking. Disruption of cellular microtubules prevents the clustering of both GABARAP and GABA(A) receptors. GABARAP also interacts with other receptors such as the glycine receptor at the postsynaptic site2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 02/03/2021

Anti-GABARAP Antibody (#AIP-016) is a highly specific antibody directed against an extracellular epitope of the rat GABA receptor associated protein. The antibody can be used in western blot analysis. It has been designed to recognize GABARAP from human, mouse and rat samples. Due to high homology with GABA receptor associated protein-like proteins the antibody will recognize GABA receptor associated protein 1 and 3 and may recognize GABA receptor associated protein 2.

For research purposes only, not for human use
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