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- Peptide (C)REARDKAVHERGH, corresponding to amino acid residues 35-47 of rat GAT-3 (Accession P31647). Intracellular, N-terminus.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysate:1,2. Anti-GABA Transporter 3 (GAT-3) Antibody (#AGT-003), (1:500).
3,4. Anti-GABA Transporter 3 (GAT-3) Antibody, preincubated with the negative control antigen.
γ-Aminobutiric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Transmission via GABA can be halted by its reuptake through GABA transporters (GATs). These membrane proteins belong to the super family of slc6 genes which encode transporters responsible for the uptake of dopamine, serotonin, norepinephrine, glycine and GABA in a Na+-dependent manner1,2.
To date, four GATs have been identified: GAT-1, GAT-2, GAT-3 and BGT-1. Like all members of the slc6 family, they have twelve transmembrane domains and short intracellular N- and C-termini1. GATs couple the transport of one GABA molecule to 2 Na+ ions and one Cl- ion3.
GAT-3 is highly expressed in the brain and is linked to epilepsy4.
Species reactivity key:
Anti-GABA Transporter 3 (GAT-3) Antibody (#AGT-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize GAT-3 from rat and mouse samples. The antibody will unlikely recognize the protein from human samples.
- Rat cerebellum lysate.
Cabrera-Pastor, A. et al. (2019) Neuropharmacology 161, 107496.