Anti-GABA Transporter 3 (GAT-3) Antibody

SLC6A11, GABT3, Na+/Cl- -dependent GABA transporter 3
    Cat #: AGT-003
    Alternative Name SLC6A11, GABT3, Na+/Cl- -dependent GABA transporter 3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    Immunogen
    • Peptide (C)REARDKAVHERGH, corresponding to amino acid residues 35-47 of rat GAT-3 (Accession P31647). Intracellular, N-terminus.
    • Anti-GABA Transporter 3 (GAT-3) Antibody
    Accession (Uniprot) Number P31647
    Gene ID 79213
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – identical.
    RRID AB_2340977.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity Not recommended for use with human samples.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
    Western blot
    • Anti-GABA Transporter 3 (GAT-3) Antibody
      Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysate:
      1,2. Anti-GABA Transporter 3 (GAT-3) Antibody (#AGT-003), (1:500).
      3,4. Anti-GABA Transporter 3 (GAT-3) Antibody, preincubated with the negative control antigen.
    References
    1. Gonzalez-Burgos, G. (2010) Adv. Pharmacol. 58, 175.
    2. Gether, U. et al. (2006) Trends Pharmacol. Sci. 27, 375.
    3. Meinild, A.K. et al. (2009) J. Biol. Chem. 284, 16226.
    4. Kristensen, A.S. et al. (2011) Pharmacol. Rev. 63, 585.
    Scientific background

    γ-Aminobutiric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Transmission via GABA can be halted by its reuptake through GABA transporters (GATs). These membrane proteins belong to the super family of slc6 genes which encode transporters responsible for the uptake of dopamine, serotonin, norepinephrine, glycine and GABA in a Na+-dependent manner1,2.

    To date, four GATs have been identified: GAT-1, GAT-2GAT-3 and BGT-1. Like all members of the slc6 family, they have twelve transmembrane domains and short intracellular N- and C-termini1. GATs couple the transport of one GABA molecule to 2 Na+ ions and one Cl- ion3.

    GAT-3 is highly expressed in the brain and is linked to epilepsy4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 18/05/2020

    Anti-GABA Transporter 3 (GAT-3) Antibody (#AGT-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize GAT-3 from rat and mouse samples. The antibody will unlikely recognize the protein from human samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Western blot citations
    1. Rat cerebellum lysate.
      Cabrera-Pastor, A. et al. (2019) Neuropharmacology 161, 107496.
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