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Anti-GABA(A) β2 Receptor Antibody

Gamma-Aminobutyric Acid Receptor Subunit Beta-2, GABA(A) receptor subunit beta-2, GABA(A) Receptor Beta 2, GABRB2

Cat #: AGA-012
Alternative Name Gamma-Aminobutyric Acid Receptor Subunit Beta-2, GABA(A) receptor subunit beta-2, GABA(A) Receptor Beta 2, GABRB2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KAGLPRHSFGRNALE, corresponding to amino acid residues 405 - 419 of rat GABRB2 (Accession P63138). Intracellular, 2nd loop.
Accession (Uniprot) Number P63138
Gene ID 25451
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, Human - identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
GABA(A) β2 Receptor Blocking Peptide (BLP-GA012)
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    1-2. Anti-GABA(A) β2 Receptor Antibody (#AGA-012), (1:200).
    3-4. Anti-GABA(A) β2 Receptor Antibody, preincubated with GABA(A) β2 Receptor Blocking Peptide (BLP-GA012).
  • Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 3) and human U-87 MG glioblastoma cell line lysate (lanes 2 and 4):
    Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 3) and human U-87 MG glioblastoma cell line lysate (lanes 2 and 4):
    1-2. Anti-GABA(A) β2 Receptor Antibody (#AGA-012), (1:200).
    3-4. Anti-GABA(A) β2 Receptor Antibody, preincubated with GABA(A) β2 Receptor Blocking Peptide (BLP-GA012).
Immunohistochemistry
  • Expression of GABA(A) Receptor Beta 2 in mouse hypothalamus.
    Expression of GABA(A) Receptor Beta 2 in mouse hypothalamus.
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections Anti-GABA(A) β2 Receptor Antibody (#AGA-012), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the periventricular hypothalamus region, showed GABA(A) Receptor Beta 2 immunoreactivity (green) in periventricular glial processes (arrows). B. Pre-incubation of the antibody with GABA(A) β2 Receptor Blocking Peptide (BLP-GA012), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = 3rd ventricle.
  • Expression of GABA(A) Receptor Beta 2 in rat hypothalamus.
    Expression of GABA(A) Receptor Beta 2 in rat hypothalamus.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections Anti-GABA(A) β2 Receptor Antibody (#AGA-012), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the periventricular hypothalamus region, showed GABA(A) Receptor Beta 2 immunoreactivity (green) in periventricular glial processes (arrows). B. Pre-incubation of the antibody with GABA(A) β2 Receptor Blocking Peptide (BLP-GA012), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = 3rd ventricle.
References
  1. Olsen, R.W. et al. (2018) Neuropharmacology, 136(Pt A): p. 10-22.
  2. Macdonald, R.L. and R.W. Olsen, et al. (1994) Annu Rev Neurosci, 17: p. 569-602.
  3. Olsen, R.W. and W. Sieghart. et al. (2008) Pharmacol Rev, 60(3): p. 243-60.
  4. Olsen, R.W. and W. Sieghart, et al. (2009) Neuropharmacology, 56(1): p. 141-8.
  5. Barki, M. et al. (2022) Gene. 809, 146021.
Scientific background

GABA receptors have two main subtypes: GABA(A) and GABA(B) receptors based on their amino acid sequence and structure. The GABA(A) receptors are ionotropic receptors while the GABA(B) receptors are metabotropic G-protein coupled receptors1.

To date, 19 GABA(A) receptor subunits have been identified: α 1-6, β 1-3, γ1-3, δ, ε, θ, p and ρ 1-34. GABA(A)receptors are heteropentameric complexes formed by combinations of different GABA(A) receptor subunits 4.

The major isoform of the GABA(A) receptor in the brain is composed of two α1, two β2, and one γ2 subunits that are encoded by the GABRA1GABRB2, and GABRG2 genes respectively, accounting for 43% of the GABA receptors in the mammalian brain 5. It is then not surprising that GABA(A) Receptor Beta 2, has been associated with several neuropsychiatric disorders such as schizophrenia, bipolar disorder, epilepsy, autism spectrum disorder, Alzheimer’s disease, frontotemporal dementia and depression 5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/01/2023

Anti-GABA(A) β2 Receptor Antibody (#AGA-012) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GABA(A) Receptor Beta 2 from rat, mouse and human samples.

For research purposes only, not for human use
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