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Anti-GABA(A) β3 Receptor Antibody

γ-Aminobutyric acid receptor subunit β3, GABAA receptor beta-3, GABRB3, EIEE43, ECA5

Cat #: AGA-013
Alternative Name γ-Aminobutyric acid receptor subunit β3, GABAA receptor beta-3, GABRB3, EIEE43, ECA5
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)GRYMGDRSIPHKK, corresponding to amino acid residues 413-425 of rat GABRB3 (Accession P63079). 2nd intracellular loop.
Accession (Uniprot) Number P63079
Gene ID 24922
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical; human – 10/13 amino acid residues identical.
RRID AB_2756594.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and LNCaP cell line lysate (lanes 3 and 6):
    Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and LNCaP cell line lysate (lanes 3 and 6):
    1-3. Anti-GABA(A) β3 Receptor Antibody (#AGA-013), (1:1000)
    4-6. Anti-GABA(A) β3 Receptor Antibody, preincubated with GABA(A) β3 Receptor Blocking Peptide (#BLP-GA013).
References
  1. Middendorp, S.J. et al. (2015) Neuropharmacology 95, 459.
  2. Pomara, N. et al. (2015) Prog. Neuropsychopharmacol. Biol. Psychiatry 56, 35.
  3. Hentschke, H. et al. (2009) J. Neurophysiol. 102, 3643.
  4. Zhang, Y. et al. (2017) Medicine (Baltimore) 96, e9273.
  5. Khom, S. et al. (2016) J. Pharmacol. Exp. Ther. 357, 580.
Scientific background

GABA(A) receptors belong to the cys-loop pentameric ligand-gated ion channel family. These receptors are inhibitory neurotransmitter receptors in the brain and in the mammalian central nervous system. They are responsible for mediating GABA action, a neurotransmitter. They are composed of five homologous subunits that are arranged around a central chloride (Cl-)-selective pore. The human genome encodes 19 different GABA(A) receptor subunits: α1-6, β1-3, γ1-3, δερ1-3, π, and θ1,2,3.

The beta-3 subunit plays a crucial role in coordinating the network rhythms in the thalamus and olfactory bulb. The protein is responsible to controlling hyperexcitability, preventing seizures. GABA(A) β3 receptor expression is detected in dendritic regions of the hippocampus and dentate gyrus. The β3 subunit is also associated with slow GABAergic current kinetics3,4.

Mutation studies of GABRB3 gene are detected in patients with infantile spasms and Lennox–Gastaut syndrome. In addition, mice lacking the β3 subunit manifest fast decay kinetics on inhibitory postsynaptic currents in thalamic reticular and neocortical neurons3,5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Anti-GABA(A) β3 Receptor Antibody (#AGA-013) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize GABRB3 from human, rat, and mouse samples.

For research purposes only, not for human use
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