Overview
- Peptide (C)HAGSTLIPMNNISMPQGE, corresponding to amino acid residues 382-399 of rat GABA(A) γ1 receptor (Accession P23574). Intracellular, C-terminus.
- Rat and mouse brain lysates (1:200-1:500).
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-GABA(A) γ1 Receptor Antibody (#AGA-016), (1:500).
3,4. Anti-GABA(A) γ1 Receptor Antibody, preincubated with GABA(A) γ1 Receptor Blocking Peptide (#BLP-GA016).
- Rat DRG.
The neurotransmitter GABA (γ-aminobutyric acid) inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells1. There are two major types of GABA receptors: the ionotropic GABAA (GABAA R) and the metabotropic GABAB receptors. GABAA R belongs to the ligand gated ion channel superfamily1,2.
The binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance, resulting in inhibition of neural activity2,3.
They are composed of heteropentamer, with all of the five subunits contributing to the pore formation. To date, eight subunit isoforms have been cloned: α, β, γ, δ, ε, π, θ, and ρ1. The native GABAA receptor, in most cases, consists of 2α, 2β and 1γ subunit. Three γ subunits genes have been identified in mammals.
mRNA of the γ1 subunit is detected in the amygdala and septum4.
