Anti-GABA(A) ρ3 Receptor (GABRR3) (extracellular) Antibody

γ-Aminobutyric acid receptor subunit ρ3, GABA(C) receptor
    Cat #: AGA-020
    Alternative Name γ-Aminobutyric acid receptor subunit ρ3, GABA(C) receptor
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
      • Peptide CLSSPKQTRIRETR, corresponding to amino acid residues 31-44 of rat GABRR3 (Accession P50573). Extracellular, N-terminus.
        Anti-GABA(A) ρ3 Receptor (GABRR3) (extracellular) Antibody
    Accession (Uniprot) Number P50573
    Gene ID 192258
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – identical; human – 9/14 amino acid residues identical.
    RRID AB_2340943.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, lci, wb
    May also work in: ifc*, ih*, ip*
      • Anti-GABA(A) ρ3 Receptor (GABRR3) (extracellular) Antibody
        Western blot analysis of rat eye (lanes 1 and 3) and ARPE-19 (lanes 2 and 4):
        1,2. Anti-GABA(A) ρ3 Receptor (GABRR3) (extracellular) Antibody (#AGA-020), (1:200).
        3,4. Anti-GABA(A) ρ3 Receptor (GABRR3) (extracellular) Antibody, preincubated with the negative control antigen.
      • Anti-GABA(A) ρ3 Receptor (GABRR3) (extracellular) Antibody
        Expression of GABA(A) ρ3 receptor in human ARPE-19 cells
        Cell surface detection of GABA(A) ρ3 receptor in intact living human retinal epithelium (ARPE-19) cells. Extracellular staining of cells using Anti-GABA(A) ρ3 Receptor (GABRR3) (extracellular) Antibody (#AGA-020), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Merge of A and live view of the cells.
      • γ-Aminobutyric acid (GABA) is the most abundant inhibitory neurotransmitter. It is involved in roughly 40% of inhibitory synapses1,2.

        GABA acts through two receptors, GABA(A) and GABA(B). To date, nineteen different GABA(A) subunits have been identified and divided in eight subunits: α (1-6), β (1-3), γ (1-3), δ, ε, ρ (1-3), θ and π. For some of the subunits, alternative splicing further increases the number of existing receptor types. They all have extracellular N- and C-termini and four transmembrane domains1.

        Three ρ subunits have been detected: GABA(A) ρ1GABA(A) ρ2 and GABA(A) ρ3. Like all GABA(A) receptors the ρ subunits also assemble into a pentameric structure forming a Cl- channel. However, in contrast to all other GABA(A) subunits they mostly form homomeric entities. The GABA(A) ρ subunits display different pharmacological characteristics and were therefore once referred to as GABA(C) receptors. GABA(A) and GABA(B) respectively respond to bicuculline and baclofen, whereas ρ subunits are insensitive to either drug3-7. In addition, ρ subunits also display different electrophysiological properties, and are significantly more sensitive to GABA3,5,6,8.

        ρ subunits are highly expressed in the retina and it was believed that they are only expressed in that area. They are however, also expressed in central and peripheral nervous systems, as well as in the gastrointestinal and cardiovascular systems1.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat GABA(A) rho-3 subunit. Anti-GABA(A) ρ3 Receptor (GABRR3) (extracellular) Antibody (#AGA-020) can be used in western blot and live cell imaging applications. It has been designed to recognize GABA(A) ρ3 from human, rat and mouse samples.

    For research purposes only, not for human use