This product is freeze dried. All water molecules have been removed.
This antibody is shipped with its antigen FREE of charge!
- Peptide CRGEREVVGPKVRK, corresponding to amino acid residues 66-79 of rat GABBR1 (Accession Q9Z0U4). Extracellular, N-terminus.
- Western blot analysis of rat brain membranes:1. Anti-GABA(B) R1 (extracellular) Antibody (#AGB-001), (1:200).
2. Anti-GABA(B) R1 (extracellular) Antibody, preincubated with the negative control antigen.
- Expression of GABA(B) receptor 1 in mouse hippocampusImmunohistochemical staining of mouse hippocampus frozen sections using Anti-GABA(B) R1 (extracellular) Antibody (#AGB-001), (1:100). A. GABBR1 staining (green) is detected in neurons in the CA3 field and in the dentate granule layer (short arrows), as well as in dendrites of CA3 pyramidal neurons (long arrows). B. Staining with mouse anti-GAP43 antibody (red) sets apart the stratum lacunosum moleculare (SLM). C. Confocal merge suggests the presence of GABBR1 in pyramidal neurons.
- 1. Bowery, N.G. and Smart, T.G. (2006) Br. J. Pharmacol. 147, S109.
- 2. Bettler, B. et al. (2004) Physiol. Rev. 84, 835.
- 3. Boller, M. and Schmidt, M. (2003) J. Neurophysiol. 89, 2035.
- 4. Robbins, M.J. et al. (2001) J. Neurosci. 21, 8043.
- 5. Margeta-Mitrovic M. et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 14649.
- 6. Margeta-Mitrovic M. et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 14643.
- 7. http://www.guidetopharmacology.org/.
- 8. http://www.uniprot.org/uniprot/Q9Z0U4.
GABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system and plays a crucial role in the modulation of neuronal activity.1,2
The GABA transmitter interacts with three types of receptors: the ionotropic receptors, GABA(A) R and GABA(C) R, and the metabotropic GABA (B) receptor, [GABA(B) R].3 The latter belongs to the G-protein coupled receptor superfamily and mediates slow synaptic inhibition in the brain and spinal cord.1,4
The functional GABA (B) receptor is a heterodimer consisting of two subunits, the GABA(B) R1 and the GABA(B) R2. These subunits were demonstrated to have complementary roles essential for the functional receptor.5,6 The GABA(B) R1 subunit was demonstrated to be important for agonist and antagonist binding, while GABA(B) R2 was shown to be essential for trafficking and for G-protein binding.
To date, eight alternatively spliced isoforms of GABA (B) R1 have been proposed. These are named 1a-1h, of which 1a and 1b are the most prominent. Only 1a, 1b, and 1c appear to act as functional subunits.6,7 They are widespread, being expressed in the testis, stomach, spinal cord, and brain. Isoform 1b is also expressed in the kidney and liver.8
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat GABA(B) receptor 1. Anti-GABA(B) R1 (extracellular) Antibody (#AGB-001) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GABBR1 from human, rat, and mouse samples.
- Activators/Agonists: small molecules