Anti-GABA(B) R1 (extracellular) Antibody

γ-Aminobutyric acid type B receptor subunit 1, GABA B receptor 1, GABABR1, Gb1, GABBR1, GPRC3A
    Cat #: AGB-001
    Alternative Name γ-Aminobutyric acid type B receptor subunit 1, GABA B receptor 1, GABABR1, Gb1, GABBR1, GPRC3A
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide CRGEREVVGPKVRK, corresponding to amino acid residues 66-79 of rat GABBR1 (Accession Q9Z0U4). Extracellular, N-terminus.
        Anti-GABA(B) R1 (extracellular) Antibody
    Accession (Uniprot) Number Q9Z0U4
    Gene ID 81657
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human, canine, bovine - identical.
    RRID AB_2039857.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity Does not recognize alternative splice 1b.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*, lci*
      • Anti-GABA(B) R1 (extracellular) Antibody
        Western blot analysis of rat brain membranes:
        1. Anti-GABA(B) R1 (extracellular) Antibody (#AGB-001), (1:200).
        2. Anti-GABA(B) R1 (extracellular) Antibody, preincubated with the negative control antigen.
      • Anti-GABA(B) R1 (extracellular) Antibody
        Expression of GABA(B) receptor 1 in mouse hippocampus
        Immunohistochemical staining of mouse hippocampus frozen sections using Anti-GABA(B) R1 (extracellular) Antibody (#AGB-001), (1:100). A. GABBR1 staining (green) is detected in neurons in the CA3 field and in the dentate granule layer (short arrows), as well as in dendrites of CA3 pyramidal neurons (long arrows). B. Staining with mouse anti-GAP43 antibody (red) sets apart the stratum lacunosum moleculare (SLM). C. Confocal merge suggests the presence of GABBR1 in pyramidal neurons.
    References
      • GABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system and plays a crucial role in the modulation of neuronal activity.1,2

        The GABA transmitter interacts with three types of receptors: the ionotropic receptors, GABA(A) R and GABA(C) R, and the metabotropic GABA (B) receptor, [GABA(B) R].3 The latter belongs to the G-protein coupled receptor superfamily and mediates slow synaptic inhibition in the brain and spinal cord.1,4

        The functional GABA (B) receptor is a heterodimer consisting of two subunits, the GABA(B) R1 and the GABA(B) R2. These subunits were demonstrated to have complementary roles essential for the functional receptor.5,6 The GABA(B) R1 subunit was demonstrated to be important for agonist and antagonist binding, while GABA(B) R2 was shown to be essential for trafficking and for G-protein binding.

        To date, eight alternatively spliced isoforms of GABA (B) R1 have been proposed. These are named 1a-1h, of which 1a and 1b are the most prominent. Only 1a, 1b, and 1c appear to act as functional subunits.6,7 They are widespread, being expressed in the testis, stomach, spinal cord, and brain. Isoform 1b is also expressed in the kidney and liver.8

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/11/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat GABA(B) receptor 1. Anti-GABA(B) R1 (extracellular) Antibody (#AGB-001) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GABBR1 from human, rat, and mouse samples.

    For research purposes only, not for human use