Anti-GALR3 (extracellular) Antibody

Galanin receptor type 3, GALNR3, GAL3R
    Cat #: AGR-013
    Alternative Name Galanin receptor type 3, GALNR3, GAL3R
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide SYYGTVRYGALELC, corresponding to amino acid residues 159-172 of mouse Galanin receptor type 3 (Accession O88853). 2nd extracellular loop.
        Anti-GALR3 (extracellular) Antibody
    Accession (Uniprot) Number O88853
    Gene ID 14429
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, human - identical.
    RRID AB_2039874.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.7 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*, lci*
      • Anti-GALR3 (extracellular) Antibody
        Western blot analysis of rat brain membrane:
        1. Anti-GALR3 (extracellular) Antibody (#AGR-013), (1:200).
        2. Anti-GALR3 (extracellular) Antibody, preincubated with the control peptide antigen.
      • Anti-GALR3 (extracellular) Antibody
        Expression of Galanin receptor 3 in rat hippocampus
        Immunohistochemical staining of rat hippocampus using Anti-GALR3 (extracellular) Antibody (#AGR-013). A. Galanin receptor 3 (green) appears in apical dendrites of pyramidal neurons in CA1 (arrows). B. MAP2 (red), a marker of axons and dendrites, is stained in the same section. GALR3 appears to be expressed in a subset of apical dendrites (arrows). DAPI is used as the counterstain (blue).
    References
      • Galanin is a neuropeptide, consisting of 29-30 amino acid residues, involved in the regulation of cognition, mood, feeding, seizure activity and pain. Galanin exerts its action via three known receptors, the Galanin receptor type 1 (GALR1), Galanin receptor type 2 (GALR2) and Galanin receptor type 3 (GALR3). The Galanin receptors belong to the G-protein coupled receptor superfamily.1-4

        Based on mRNA, the GALR1 is predominantly distributed in the central nervous system, spinal cord and dorsal root ganglia while GALR2 is abundant in both central and peripheral nervous systems. GALR3 mRNA is distributed in peripheral tissues such as the heart, spleen, and testis. However, its expression in the brain is still controversial.4,5

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-GALR3 (extracellular) Antibody (#AGR-013) is a highly specific antibody directed against an epitope of the mouse Galanin receptor 3. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GALR3 from mouse, rat, and human samples.

    For research purposes only, not for human use