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Anti-GAP43 Antibody

Axonal membrane protein GAP-43, Growth-associated protein 43, Neuromodulin, Neural phosphoprotein B-50

Cat #: ANR-016
Alternative Name Axonal membrane protein GAP-43, Growth-associated protein 43, Neuromodulin, Neural phosphoprotein B-50
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)KPKESARQDEGKED, corresponding to amino acid residues 205-218 of rat GAP43 (Accession P07936). C-terminus.
Accession (Uniprot) Number P07936
Gene ID 29423
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical; human – 13/14 amino acid residues identical.
RRID AB_2756686.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    1,2 Anti-GAP43 Antibody (#ANR-016), (1:400).
    3,4. Anti-GAP43 Antibody, preincubated with GAP43 Blocking Peptide (#BLP-NR016).
Immunohistochemistry
  • Expression of GAP43 in rat hippocampus
    Expression of GAP43 in rat hippocampus
    Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GAP43 Antibody (#ANR-016), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. GAP43 staining (green) appears in a layer bordering granule layer (G), (arrow). B. Calbindin D28k staining (red) is detected in granule layer (G) neurons. C. Merge of the two images demonstrates distinct spatial localizations in the hippocampal dentate gyrus. Cell nuclei are stained with DAPI (blue).
References
  1. Li, Y. et al. (2017) Braz. J. Med. Biol. Res. 50, e5561.
  2. Grasselli, G. and Strata, P. (2013) Front. Neural Circuits. 7, 25.
Scientific background

Growth associated protein 43 (GAP43) is a neuron specific phosphoprotein that plays an important role in the process of nerve growth, development, regeneration and axonal remodeling. GAP43 belongs to the calmodulin-binding protein family and is highly distributed in the presynaptic membrane1.

GAP43 is expressed in developing neurons, especially in the growth cone. In mature neurons, GAP43 is detected in regenerating nerves. In brain injury, neurons exhibit sprouting of the lateral branches and responsive regeneration of the axon accompanied by increased expression of GAP43. GAP43 expression is used as an important tool for evaluating axon injury and the regenerative response in the mature central nervous system (CNS)1,2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 06/11/2022

Anti-GAP43 Antibody (#ANR-016) is a highly selective antibody directed against an epitope of rat Neuromodulin. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GAP43 from human, mouse, and rat samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

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