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Anti-GFR alpha 3 (extracellular) Antibody

GFRA3, GFRα3, GDNF family receptor α3, GDNF receptor alpha 3

Cat #: ANT-023
Alternative Name GFRA3, GFRα3, GDNF family receptor α3, GDNF receptor alpha 3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide CDRLRKAYGEA(S)SG, corresponding to amino acid residues 172-185 of mouse GFRA3 (Accession O35118). Extracellular, N-terminus.
Accession (Uniprot) Number O35118
Gene ID 14587
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat, human – 13/14 amino acid residues identical.
RRID AB_2756710.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*, lci*
Western blot
  • Western blot analysis of mouse brain lysate:
    Western blot analysis of mouse brain lysate:
    1. Anti-GFR alpha 3 (extracellular) Antibody (#ANT-023), (1:200).
    2. Anti-GFR alpha 3 (extracellular) Antibody, preincubated with GFR α 3 (extracellular) Blocking Peptide (#BLP-NT023).
  • Western blot analysis of rat brain lysate:
    Western blot analysis of rat brain lysate:
    1. Anti-GFR alpha 3 (extracellular) Antibody (#ANT-023), (1:200).
    2. Anti-GFR alpha 3 (extracellular) Antibody, preincubated with GFR α 3 (extracellular) Blocking Peptide (#BLP-NT023).
References
  1. Shang, H.Q. et al. (2016) J. Headache Pain 17, 81.
  2. Yoshida, N. et al. (2011) Mol. Pain 7, 41.
  3. Nivlet, L. et al. (2016) J. Mol. Endocrinol. 56, 77.
  4. Parkash, V. and Goldman, A. (2009) Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 65, 551.
Scientific background GFRα3 (GDNF Family Receptor α3) belongs to the glial cell line derived neurotrophic factor (GDNF) family of receptors which contains four members GFRα1-4. The GDNF family of ligands (GFLs)- GDNF, Neurturin, Artemin, and Persephin bind to co-receptors GFRα1–4 and activate Ret receptor tyrosine kinase. GDNF-family receptor α is glycosyl-phosphatidyl­inositol anchored to the cell surface. GFRα3 structure contains three homologous domains (D1, D2 and D3) and a C-terminal extension1-4.

GFRα3 is a highly selective receptor for Artemin (Artn). Artemin is a vasculature-derived neurotrophic factor that modulates the development and function of sensory neurons and participates in the pathophysiology of peripheral inflammation and pain hyperalgesia.

In adults, GFRα3 is restricted to neurons of the peripheral nervous system. The protein is expressed by a subpopulation of nociceptive sensory neurons which also express the Ret receptor tyrosine kinase, TRPV1, TRPA11,2,3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Anti-GFR alpha 3 (extracellular) Antibody (#ANT-023) is a highly specific antibody directed against an epitope of mouse GDNF receptor α3. The antibody can be used in western blot analysis. It has been designed to recognize GFR α3 from human, mouse, and rat samples.

For research purposes only, not for human use
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