Anti-Ghrelin Receptor (GHSR) (extracellular)-FITC Antibody

Growth hormone secretagogue receptor type 1, GHS-R, GH-releasing peptide receptor, GHRP
    Cat #: AGR-031-F
    Alternative Name Growth hormone secretagogue receptor type 1, GHS-R, GH-releasing peptide receptor, GHRP
  • Lyophilized Powder
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide CRATEFAVRSG, corresponding to amino acid residues 197-207 of rat Ghrelin receptor (Accession O08725). 2nd extracellular loop.
    • Anti-Ghrelin Receptor (GHSR) (extracellular)-FITC Antibody
    Accession (Uniprot) Number O08725
    Gene ID 84022
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human – 10 out of 11 amino acid residues identical.
    RRID AB_2756628.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label Fluorescein isothiocyanate (FITC).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGR-031), and direct flow cytometry (labeled antibody).
    Applications: fc
    May also work in: ic*, lci*
    Direct flow cytometry
    • Anti-Ghrelin Receptor (GHSR) (extracellular)-FITC Antibody
      Cell surface detection of Ghrelin receptor in live intact mouse TK-1 T-cell lymphoma cells:
      ___ Cells.
      ___ Cells + rabbit IgG isotype control-FITC.
      ___ Cells + Anti-Ghrelin Receptor (GHSR) (extracellular)-FITC Antibody (#AGR-031-F), (2.5 µg).
    • Anti-Ghrelin Receptor (GHSR) (extracellular)-FITC Antibody
      Cell surface detection of Ghrelin receptor in live intact human THP-1 monocytic leukemia cells:
      ___ Cells.
      ___ Cells + rabbit IgG isotype control-FITC.
      ___ Cells + Anti-Ghrelin Receptor (GHSR) (extracellular)-FITC Antibody (#AGR-031-F), (5 µg).
    References
    1. Sato, T. et al. (2012) J. Biochem. 151, 119.
    2. Kojima, M. et al. (1999) Nature 402, 656.
    3. Albarran-Zeckler, R.G. et al. (2011) Peptides 32, 2229.
    4. Seim, I. et al. (2011) Mol. Cell. Endo. 340, 3.
    5. Takahashi, K. et al. (2006) Cancer Res. 66, 9408.
    Scientific background

    Ghrelin is a 28 amino acid peptide, acylated on a serine residue which is essential for its biological activity1,2. In rodents, an additional form of the peptide could be purified. The peptide is released from the stomach and exerts its effect on the ghrelin receptor (GHS-R). GHS-R is a member of the G-protein coupled receptor (GPCR) superfamily. Like all members, the receptor has seven transmembrane spanning domains and upon activation it stimulates phospholipase C, thereby increasing IP3 and intracellular Ca2+ levels1,3.

    GHS-R is expressed in the hypothalamus, pituitary and hippocampus1,3.

    Some of the actions of ghrelin include the stimulation of growth hormone release, food intake, fat accumulation, regulation of memory and learning, activation of reward-related pathways and neuroprotection to name a few1,3.

    GHS-R 1b is a truncated form of the full length receptor which is also expressed. Although its exact functions are unknown, it is believed that it may be involved in downregulating signaling from the full length receptor4. In addition, it is overexpressed in lung cancer where it dimerizes with neurotensin receptor4,5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 05/03/2020

    Anti-Ghrelin Receptor (GHSR) (extracellular) Antibody (#AGR-031) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize GHSR from human, mouse, and rat samples.

    Anti-Ghrelin Receptor (GHSR) (extracellular)FITC Antibody (#AGR-031-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

    For research purposes only, not for human use
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