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Anti-GLP1R (extracellular)-FITC Antibody

Glucagon-like peptide 1 receptor, GLP-1 receptor

Cat #: AGR-021-F
Alternative Name Glucagon-like peptide 1 receptor, GLP-1 receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
  • Peptide (C)KYLYEDEG(S)WTRNS, corresponding to amino acid residues 288-301 of rat GLP1R (Accession P32301). 2nd extracellular, loop.
Accession (Uniprot) Number P32301
Gene ID 25051
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse, human - 13/14 amino acid residues identical.
RRID AB_2756626.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label Fluorescein isothiocyanate (FITC).
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGR-021), and direct flow cytometry (labeled antibody).
Applications: fc, lci
May also work in: ic*
Direct flow cytometry
  • Cell surface detection of GLP1R in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of GLP1R in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    Cells + rabbit IgG isotype control-FITC.
    Cells + Anti-GLP1R (extracellular)-FITC Antibody (#AGR-021-F), (5 µg).
  • Cell surface detection of GLP1R in live intact human MEG-01 megakaryoblastic leukemia cells:
    Cell surface detection of GLP1R in live intact human MEG-01 megakaryoblastic leukemia cells:
    ___ Cells.
     Cells + rabbit IgG isotype control-FITC.
     Cells + Anti-GLP1R (extracellular)-FITC Antibody (#AGR-021-F), (2.5 µg).
  1. Donath, M.Y. et al. (2003) J. Mol. Med. 81, 455.
  2. Buteau, J. (2008) Diabetes Metab. 34, S73.
  3. Holst, J.J. (2007) Physiol. Rev. 87, 1409.
  4. Harkavyi, A. and Whitton, P.S. (2010) Br. J. Pharmacol. 159, 495.
Scientific background

Glucagon-like peptide 1 (GLP-1) is a hormone secreted by the intestine following a meal1. This hormone is known to enhance the secretion of insulin, important to regulate glucose blood levels2,3. In pancreatic b-cells, other than stimulating insulin secretion, GLP-1 action is also important for b-cell mass expansion2.

GLP-1 exerts its biological functions through Glucagon-like peptide 1 receptor (GLP-1 receptor), a member of the G-protein coupled receptor superfamily. Like all members this receptor spans the membrane seven times. GLP-1 receptor couples with Gs, thereby stimulates adenylate cyclase which consequently leads to an increase in cAMP2.

The receptor is expressed in various tissues, namely the pancreas, adipose tissue, muscle, heart, gastrointestinal tract and liver. GLP-1 receptor is also found in the central nervous system where it provides a neuroprotective effect4.

The glucagon-like peptide 1 system may serve as a therapeutic treatment in diabetes mellitus, in order to maintain b-cell mass expansion2. In addition, the receptor may serve as a therapy target in various neurodegenerative diseases like Alzheimer’s and Parkinson’s disease4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 02/01/2022

Anti-GLP1R (extracellular) Antibody (#AGR-021) is a highly specific antibody directed against an epitope of the rat Glucagon-like peptide 1 receptor. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and live cell imaging applications. It has been designed to recognize GLP1R from human, rat, and mouse samples.

Anti-GLP1R (extracellular)-FITC Antibody (#AGR-021-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

For research purposes only, not for human use



Scientific Background

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