Anti-GPR119 Antibody

Glucose-dependent insulinotropic receptor, GPCR2
    Cat #: AGR-032
    Alternative Name Glucose-dependent insulinotropic receptor, GPCR2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
      • Peptide CDMLK IASVHSQHIR, corresponding to amino acid residues 186-200 of rat GPR119 (Accession Q7TQN8). 2nd intracellular loop.
        Anti-GPR119 Antibody
    Accession (Uniprot) Number Q7TQN8
    Gene ID 302813
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical.
    RRID AB_2756629.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
      • Anti-GPR119 Antibody
        Western blot analysis of rat pancreas (lanes 1 and 3) and mouse MS1 cells (lanes 2 and 4):
        1,2. Anti-GPR119 Antibody (#AGR-032), (1:400).
        3,4. Anti-GPR119 Antibody, preincubated with the negative control antigen.
    References
    • 1. Griffin, G. (2006) Methods for identification of modulators of OSGPR116 activity.
    • 2. Fredriksson, R. et al. (2003) FEBS Lett. 554, 381.
    • 3. Overton, H.A. et al. (2007) Br, J, Pharmacol, 153, S76.
    • 4. Ohishi, T. and Yoshida, S. (2012) Expert Opin. Investig. Drugs 21, 321.
    • 5. Hansen, H.S. et al. (2012) Trends Pharmacol. Sci. 33, 374.
      • Glucose-dependent insulinotropic receptor (GPR119) is a rhodopsin-type member of the G protein-coupled receptor (GPCRs) superfamily. Like all members, it has seven transmembrane spanning domains, an extracellular N-terminus and intracellular C-terminus. Activation of the receptor leads to a rise in intracellular cAMP – suggesting a stimulation of adenylate cyclase, possibly by Gαs coupling. It is expressed mostly in enteroendocrine cells of the gastrointenstinal tract and b-cells of the pancreas. It was stipulated that GPR119 is a close relative to the cannabinoid receptors1,2.

        Phospholipids and fatty acid amides are endogenous ligands for GPR119. Notably, oleoethanolamide (OEA) seems to be an important agonist. Stimulation of GPR119 by its agonists causes b-cells to release insulin and enteroendocrine cells to release glucagon-like peptide-1 (GLP-1); this attribute makes GPR119 a strong therapeutic candidate for type-2 diabetes and obesity3,4. Furthermore, its ability to release GLP-1 upon stimulation by dietary fat has gained it the title "fat sensor"5 .

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 18/08/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Glucose-dependent insulinotropic receptor. Anti-GPR119 Antibody (#AGR-032) can be used in western blot analysis. It has been designed to recognize GPR119 from rat, mouse and human samples.

    For research purposes only, not for human use