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Anti-GLUT1 Antibody

Glucose transporter 1, Facilitated glucose transporter member 1, SLC2A1

Cat #: AGT-021
Alternative Name Glucose transporter 1, Facilitated glucose transporter member 1, SLC2A1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RQGGASQSDKTPEE, corresponding to amino acid residues 468-481 of human Glucose transporter 1 (Accession P11166). Intracellular, C-terminus.
Accession (Uniprot) Number P11166
Gene ID 6513
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse - identical.
RRID AB_2756641.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1, 2. Anti-GLUT1 Antibody (#AGT-021), (1:500).
    3, 4. Anti-GLUT1 Antibody, preincubated with GLUT1 Blocking Peptide (#BLP-GT021).
Immunohistochemistry
  • Expression of Glucose Transporter 1 in mouse striatum and wall of lateral ventricle
    Expression of Glucose Transporter 1 in mouse striatum and wall of lateral ventricle
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-GLUT1 Antibody (#AGT-021), (1:400), followed by donkey-anti-rabbit-Cy3 secondary antibody (red). GLUT1 staining appears in blood vessels (right-pointing arrows) in the striatum (ST) and in the wall of the lateral ventricle (LV, left-pointing arrow). Cell nuclei were stained with DAPI (blue).
References
  1. Olson, A.L. et al. (1996) Annu. Rev. Nutr 16, 235.
  2. Pardridge, W.M. et al. (1990) J. Biol. Chem. 265, 18035.
  3. Klepper, J. et al. (1999) Neurochem. Res. 24, 587.
  4. Leen, W.G. et al. (2014) J. Neurol. 261, 589.
  5. Amann, T. et al. (2011) Mol. Membr. Biol. 28, 182.
Scientific background

Glucose transporter 1 (GLUT1) belongs to the major facilitator superfamily (MFS), one of the largest and most ubiquitous secondary transporter superfamilies. GLUT1, encoded by SLC2A1, mediates the basal-level cellular uptake of glucose into many tissues. GLUT1 contains 12 membrane-spanning domains with both the amino and carboxyl termini oriented intracellularly. In addition, a single extracellular N-linked glycosylation site is present1.

GLUT1 is widely expressed, but it is most abundant in fibroblasts, erythrocytes, and endothelial cells with low levels of expression in muscle, liver, and adipose tissue2.

Inactivating mutations of GLUT1, resulting in compromised transport activities for glucose, are associated with diseases as a result of lack of energy supply to the brain3. GLUT1 deficiency syndrome (also known as De Vivo syndrome) is characterized by a spectrum of symptoms including early-onset seizures, microcephaly and retarded development4. In addition, elevated expression levels of GLUT1 have been observed in several cancer types, identifying GLUT1 as an important prognostic indicator for tumorigenesis5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Anti-GLUT1 Antibody (#AGT-021) is a highly specific antibody directed against an epitope of the human Glucose Transporter 1 protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GLUT1 from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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