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Home › Products › Transporters, Exchangers and Pumps › Glucose Transporters › Facilitative Glucose Transporters (GLUTs) › Antibodies

Certificate of Analysis

Anti-GLUT1 Antibody

Glucose transporter 1, Facilitated glucose transporter member 1, SLC2A1

Cat #: AGT-021
Alternative Name Glucose transporter 1, Facilitated glucose transporter member 1, SLC2A1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RQGGASQSDKTPEE, corresponding to amino acid residues 468-481 of human Glucose transporter 1 (Accession P11166). Intracellular, C-terminus.
Accession (Uniprot) Number P11166
Gene ID 6513
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse - identical.
RRID AB_2756641.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Rat and mouse brain membranes (1:500-1:2000).
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1, 2. Anti-GLUT1 Antibody (#AGT-021), (1:500).
    3, 4. Anti-GLUT1 Antibody, preincubated with GLUT1 Blocking Peptide (#BLP-GT021).
Immunohistochemistry
  • Mouse brain sections (1:400).
Scientific background

Glucose transporter 1 (GLUT1) belongs to the major facilitator superfamily (MFS), one of the largest and most ubiquitous secondary transporter superfamilies. GLUT1, encoded by SLC2A1, mediates the basal-level cellular uptake of glucose into many tissues. GLUT1 contains 12 membrane-spanning domains with both the amino and carboxyl termini oriented intracellularly. In addition, a single extracellular N-linked glycosylation site is present1.

GLUT1 is widely expressed, but it is most abundant in fibroblasts, erythrocytes, and endothelial cells with low levels of expression in muscle, liver, and adipose tissue2.

Inactivating mutations of GLUT1, resulting in compromised transport activities for glucose, are associated with diseases as a result of lack of energy supply to the brain3. GLUT1 deficiency syndrome (also known as De Vivo syndrome) is characterized by a spectrum of symptoms including early-onset seizures, microcephaly and retarded development4. In addition, elevated expression levels of GLUT1 have been observed in several cancer types, identifying GLUT1 as an important prognostic indicator for tumorigenesis5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
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Last update: 11/04/2021

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For research purposes only, not for human use

Specifications

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Scientific Background

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    Control Product

    Save $45 off the Blocking Peptide when purchased together with the antibody
    • A Blocking peptide for Anti-GLUT1 Antibody (#AGT-021). Intended to be used as a negative control.


      Price: $245

      GLUT1 Blocking Peptide (#BLP-GT021)
      Add peptide to cart

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