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Anti-GLUT1 (extracellular)-APC Antibody

Glucose transporter 1, Facilitated glucose transporter member 1, SLC2A1, DYT9

Cat #: AGT-041-APC
Alternative Name Glucose transporter 1, Facilitated glucose transporter member 1, SLC2A1, DYT9
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)EEFYNQTWNHRYGES, corresponding to amino acid residues 41-55 of rat GLUT1 (Accession P11167). 1st extracellular loop.
Accession (Uniprot) Number P11167
Gene ID 24778
Homology Not recommended for mouse and rat samples.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Label Allophycocyanin (APC)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGT-041), and direct flow cytometry (labeled antibody).
Applications: fc, lci
Direct flow cytometry
  • Cell surface detection of GLUT1 by direct flow cytometry in live intact human Jurkat T-cell leukemia cells:
    Cell surface detection of GLUT1 by direct flow cytometry in live intact human Jurkat T-cell leukemia cells:
    ___ Cells.
    ___ Cells + Rabbit IgG Isotype control-APC (#RIC-001-APC).
    ___ Cells + Anti-GLUT1 (extracellular)-APC Antibody (#AGT-041-APC), (5 µg).
  • Cell surface detection of GLUT1 by direct flow cytometry in live intact mouse TK-1 T-cell lymphoma cells:
    Cell surface detection of GLUT1 by direct flow cytometry in live intact mouse TK-1 T-cell lymphoma cells:
    ___ Cells.
    ___ Cells + Rabbit IgG Isotype control-APC (#RIC-001-APC).
    ___ Cells + Anti-GLUT1 (extracellular)-APC Antibody (#AGT-041-APC), (5 µg).
  • Cell surface detection of GLUT1 by direct flow cytometry in live intact mouse J774 macrophage cells:
    Cell surface detection of GLUT1 by direct flow cytometry in live intact mouse J774 macrophage cells:
    ___ Cells.
    ___ Cells + Rabbit IgG Isotype control-APC (#RIC-001-APC).
    ___ Cells + Anti-GLUT1 (extracellular)-APC Antibody (#AGT-041-APC), (5 µg).
References
  1. Olson, A.L. et al. (1996) Annu. Rev. Nutr 16, 235.
  2. Pardridge, W.M. et al. (1990) J. Biol. Chem265, 18035.
  3. Klepper, J. et al. (1999) Neurochem. Res. 24, 587.
  4. Leen, W.G. et al. (2014) J. Neurol. 261, 589.
  5. Amann, T. et al. (2011) Mol. Membr. Biol28, 182.
Scientific background

Glucose transporter 1 (GLUT1) belongs to the major facilitator superfamily (MFS). It is encoded by SLC2A1, and mediates basal-level cellular uptake of glucose into many tissues. GLUT1 contains 12 membrane-spanning domains with both the amino and carboxyl termini oriented intracellularly. In addition, a single extracellular N-linked glycosylation site is present1.

GLUT1 is widely expressed, but it is most abundant in fibroblasts, erythrocytes, and endothelial cells with low levels of expression in muscle, liver, and adipose tissue2.

Inactivating mutations of GLUT1, resulting in compromised transport activities for glucose, are associated with diseases as a result of lack of energy supply to the brain3. GLUT1 deficiency syndrome (also known as De Vivo syndrome) is characterized by a spectrum of symptoms including early-onset seizures, microcephaly and retarded development4. In addition, elevated expression levels of GLUT1 have been observed in several cancer types, identifying GLUT1 as an important prognostic indicator for tumorigenesis5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 10/01/2023

Anti-GLUT1 (extracellular) Antibody (#AGT-041) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize GLUT1 from mouse, rat and human samples.

Anti-GLUT1 (extracellular)-APC Antibody (#AGT-041-APC) is directly conjugated to Allophycocyanin (APC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

For research purposes only, not for human use
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