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Anti-GLUT4-ATTO Fluor-594 Antibody

Glucose transporter 4, Facilitated glucose transporter member 4, Insulin-responsive glucose transporter type 4, SLC2A4

Cat #: AGT-024-AR
Alternative Name Glucose transporter 4, Facilitated glucose transporter member 4, Insulin-responsive glucose transporter type 4, SLC2A4
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KPSTELEYLGPDE, corresponding to amino acid residues 495-507 of human Glucose Transporter 4 (Accession P14672). Intracellular, C-terminus.
Accession (Uniprot) Number P14672
Gene ID 6517
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse – identical.
RRID AB_2756646.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 – 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody #AGT-024), and Immunohistochemical staining (labeled antibody).
Applications: if, ih
May also work in: ic*
Immunohistochemistry
  • Expression of Glucose Transporter 4 in rat olfactory bulb
    Expression of Glucose Transporter 4 in rat olfactory bulb
    Immunohistochemical staining of rat olfactory bulb free floating frozen sections using Anti-GLUT4-ATTO Fluor-594 Antibody (#AGT-024-AR), (1:40). GLUT4 immunoreactivity (red) is strongly detected in some glomeruli (vertical arrows). Lower immunoreactivity in other glomeruli (horizontal arrows) is also observed. Nuclei are stained using DAPI as the counterstain.
References
  1. Lacombe, V.A. (2014) ISRN Vet Sci. 2014, 409547.
  2. Stöckli, J. et al. (2011) J. Cell Sci. 124, 41472.
Scientific background

The GLUT protein family is a member of the major facilitator superfamily of membrane transporters and is encoded by the SLC2 genes. GLUT proteins contain 12 membrane-spanning domains, an N-linked glycosylation site, and intracellular NH2 and COOH termini. In addition, there are several conserved residues and motifs designated “sugar transporter signatures”.

To date, 14 GLUT isoforms have been identified and divided into 3 different classes based on sequence similarity and structural and functional characteristics. GLUT4 together with GLUT 1-3 and 14 belong to Class I.

GLUT4 is an insulin-regulated glucose transporter responsible for insulin-regulated glucose uptake into fat and muscle cells. GLUT4 translocates from intracellular stores to the cell surface, from an inactive site to an active one in order to enhance glucose uptake1.

GLUT4 is expressed mainly in adipose tissue and skeletal muscle. It is also detected in brain, kidney and intestine.

There is no evidence for mutation in GLUT4 that is linked to metabolic disease in humans. However, GLUT4 polymorphisms are rare and equally common in normoglycaemic individuals and type 2 diabetics. General decrease in GLUT4 levels show signs of diabetes, while a disruption in GLUT4 expression in muscle or adipose tissue might contribute to the development of whole-body insulin resistance in humans2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Anti-GLUT4 Antibody (#AGT-024) is a highly specific antibody directed against an epitope of the human Glucose Transporter 4 protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GLUT4 from human, rat, and mouse samples.

Anti-GLUT4-ATTO Fluor-594 Antibody (#AGT-024-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-GLUT4-ATTO Fluor-594 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

For research purposes only, not for human use
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