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Anti-GnRH Receptor (extracellular) Antibody

Gonadotropin-releasing hormone receptor, GnRHR, Leutinizing hormone releasing hormone receptor, LHRHR, Luliberin receptor

Cat #: AGR-030
Alternative Name Gonadotropin-releasing hormone receptor, GnRHR, Leutinizing hormone releasing hormone receptor, LHRHR, Luliberin receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)NNASLEQDQNH, corresponding to amino acid residues 3-13 of rat GnRHR (Accession P30969). Extracellular, N-terminus.
Accession (Uniprot) Number P30969
Gene ID 81668
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical.
RRID AB_11219141.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Not recommended for use with human samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat ovary (lanes 1 and 3) and pituitary (lanes 2 and 4) lysates:
    Western blot analysis of rat ovary (lanes 1 and 3) and pituitary (lanes 2 and 4) lysates:
    1,2. Anti-GnRH Receptor (extracellular) Antibody (#AGR-030), (1:200).
    3,4. Anti-GnRH Receptor (extracellular) Antibody, preincubated with GnRH Receptor (extracellular) Blocking Peptide (#BLP-GR030).
Immunohistochemistry
  • Expression of GnRHR in rat ovary
    Expression of GnRHR in rat ovary
    Immunohistochemical staining of rat ovary paraffin-embedded sections using Anti-GnRH Receptor (extracellular) Antibody (#AGR-030). GnRHR labeling appears in the stroma surrounding the follicles. The stroma is a richly cellular connective tissue with scattered smooth muscle fibers. The theca layer is stained as well. Hematoxilin is used as the counterstain.
Indirect flow cytometry
  • Cell surface detection of GnRHR by indirect flow cytometry in live intact mouse TK1 T-cell lymphoma cells:
    Cell surface detection of GnRHR by indirect flow cytometry in live intact mouse TK1 T-cell lymphoma cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-APC.
    ___ Cells + Anti-GnRH Receptor (extracellular) Antibody (#AGR-030), (2.5μg) + goat-anti-rabbit-APC.
References
  1. Naor, Z. (2009) Front. Neuroendocrinol. 30, 10.
  2. Millar, R.P. et al. (2004) Endocr. Rev. 25, 235.
  3. Tello, J.A. et al. (2005) Endocrinology 146, 4061.
  4. Bliss, S.P. et al. (2010) Front. Neuroencrinol. 31, 322.
  5. Cheung, L.W. and Wong, A.S. (2008) FEBS J. 275, 5479.
Scientific background

Gonadotropin-releasing hormone (GnRH) or Luteinizing hormone releasing hormone (LHRH) is a decapeptide synthesized and released from neurons in the hypothalamus and stimulates the synthesis and release of gonadotropin hormone (or luteinizing hormone) and follicle stimulating hormone from the pituitary gland1. GnRH is expressed as various variants, and most vertebrates express more than one form of GnRH1-3.

GnRH and its variants bind and activate GnRH receptor (GnRHR), a member of the G-protein coupled receptor (GPCR) superfamily. Three forms of the receptor have been identified in vertebrates (types I, II and III). Like all members, GnRHR has seven transmembrane domains and an extracellular N-terminus and an intracellular C-terminal tail. The receptor also has a number of glycosylation sites in its extracellular loops. In addition, the C-terminal tail is unusually short and lacks consensus sites known to undergo post-translational modifications like phosphorylation. In consequence, this affects the receptor’s desensitization and internalization4.

Expression of GnRHR is detected in the gonadotrope and various tissues like the breast, gonads, prostate and uterus4,5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 02/08/2022

Anti-GnRH Receptor (extracellular) Antibody (#AGR-030) is a highly specific antibody directed against an epitope of rat GnRHR. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope, and can potentially be used for detecting the protein in living cells. It has been designed to recognize GnRHR from mouse and rat samples. It is not suited to recognize GnRHR from human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

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