Overview
- Peptide (C)DTLYMVHMAAYTEEG, corresponding to amino acid residues 584-598 of rat GP130 (Accession P40190). Extracellular, N-terminus.
Western blot analysis of mouse brain membrane (lanes 1 and 5), rat brain lysate (lanes 2 and 6), rat brain membrane (lanes 3 and 7) and rat uterus lysate (lanes 4 and 8):1-3. Anti-GP130 (extracellular) Antibody (#ALR-023), (1:400).
4. Anti-GP130 (extracellular) Antibody (1:200).
5-8. Anti-GP130 (extracellular) Antibody, preincubated with GP130 (extracellular) Blocking Peptide (#BLP-LR023).
Expression of GP130/CD130 in rat subfornical organ (SFO).Immunohistochemical staining of perfusion-fixed frozen rat brain sections Anti-GP130 (extracellular) Antibody (#ALR-023), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. GP130/CD130 immunoreactivity (green) appears in the SFO (arrows). B. Pre-incubation of the antibody with GP130 (extracellular) Blocking Peptide (BLP-LR023), suppressed staining. Cell nuclei are stained with DAPI (blue). FX = fornix; V = cerebral ventricle.
Cell surface detection of GP130/CD130 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-GP130 (extracellular) Antibody (#ALR-023), (2.5μg) + goat-anti-rabbit-FITC.
- Kurth, I. et al. (2000) J. Immunol. 164, 273.
- Kurth, I. et al. (1999) J. Immunol. 162, 1480.
- Timmermann, A. et al. (2002) Eur. J. Biochem. 269, 2716.
- Xu, S. et al. (2013) Expert Opin. Ther. Targets 17, 1303.
- Jones, S.A. et al. (2011) J. Clin. Invest. 121, 3375.
Interleukins (ILs) are key mediators in the regulation and coordination of the immune response. Glycoprotein 130 (gp130) belongs to the family of class I cytokine receptors and is the common signal transducing receptor subunit of the IL-6-type cytokines: IL-6, IL-11, leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), oncostatin M (OSM), and cardiotrophin-11.The inflammatory cytokines IL-6 and IL-11 induce gp130 homodimerization after binding to their specific α receptors, which leads to the activation of the Janus kinase/STAT signal transduction pathway2. Gp130 Heterodimerizes with LIFR in response to LIF, CNTF, OSM, or CT13.
The extracellular part of gp130 contains an Ig-like domain (D1) followed by single cytokine binding module (CBM) (D2 and D3) and three fibronectin type III-like (FNIII) domains (D4, D5, and D6)3.
Gp130 mRNA is ubiquitously expressed in the human body, exhibiting highest levels in saphenous vein, pericardium, ovary, omental adipose tissue, peritoneum, spleen lymph nodes, and trigeminal ganglia4.
Dysregulation of gp130 expression, activation, or associated signaling pathways are implicated in a variety of human diseases including cancer, rheumatoid arthritis, Castleman’s disease, cardiac myxoma, Crohn’s disease, psoriasis, asthma, amyotrophic lateral sclerosis (CNTF), diabetes, atherosclerosis, systemic lupus, obesity, and postmenopausal osteoporosis5.
Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat Membrane glycoprotein 130. Anti-GP130 (extracellular) Antibody (#ALR-023) can be used in western blot analysis. It has been designed to recognize GP130 from mouse, rat and human samples.
Powered by Bioz
