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Anti-GPR108 (extracellular) Antibody

G-Protein Coupled Receptor 108, Lung Seven Transmembrane Receptor 2, LUSTR2

Cat #: AGR-088
Alternative Name G-Protein Coupled Receptor 108, Lung Seven Transmembrane Receptor 2, LUSTR2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KDLQVQVRKYGEQK, corresponding to amino acid residues 124 - 137 of mouse GPR108 (Accession Q91WD0). Extracellular, N-term.
Accession (Uniprot) Number Q91WD0
Gene ID 78308
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, human – identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
GPR108 (extracellular) Blocking Peptide (BLP-GR088)
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    1-2. Anti-GPR108 (extracellular) Antibody (#AGR-088), (1:200).
    3-4. Anti-GPR108 (extracellular) Antibody, preincubated with GPR108 (extracellular) Blocking Peptide (BLP-GR088).
  • Western blot analysis of mouse TK-1 T-cell lymphoma cell line lysate (lanes 1 and 4), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 5) and mouse BV-2 microglia cell line lysate (lanes 3 and 6):
    Western blot analysis of mouse TK-1 T-cell lymphoma cell line lysate (lanes 1 and 4), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 5) and mouse BV-2 microglia cell line lysate (lanes 3 and 6):
    1-3. Anti-GPR108 (extracellular) Antibody (#AGR-088), (1:200).
    4-6. Anti-GPR108 (extracellular) Antibody, preincubated with GPR108 (extracellular) Blocking Peptide (BLP-GR088).
Immunohistochemistry
  • Expression of GPR108 in mouse red nucleus.
    Expression of GPR108 in mouse red nucleus.
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR108 (extracellular) Antibody (#AGR-088), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. GPR108 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with GPR108 (extracellular) Blocking Peptide (BLP-GR088), suppressed staining. Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of GPR108 by indirect flow cytometry in live intact mouse BV-2 microglia cell line:
    Cell surface detection of GPR108 by indirect flow cytometry in live intact mouse BV-2 microglia cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-GPR108 (extracellular) Antibody (#AGR-088), (2.5μg) + goat-anti-rabbit-FITC.
References
  1. Fredriksson, R., et al. (2003) Mol Pharmacol, 63(6): p. 1256-72.
  2. Watkins, L.R. and C. Orlandi. (2020) Genes (Basel), 11(6).
  3. Stadel, J.M., S. Wilson, and D.J. Bergsma. (1997)  Trends Pharmacol Sci, 18(11): p. 430-7.
  4. Marchese, A., et al. (1999) Trends Pharmacol Sci20(9): p. 370-5.
  5. Edgar, A.J. (2007) DNA Seq, 18(3): p. 235-41.
  6. Halsey, T.A., et al. (2007) Genome Biol, 8(6): p. R104.
  7. Dong, D., et al. (2018) PLoS One, 13(10): p. e0205303.
  8. Dudek, A.M. et al. (2020) Mol. Ther. 28, 367.
Scientific background

G protein coupled receptors (GPCR or GPR) comprise a large superfamily of receptors characterized by a seven transmembrane domain structure and an ability to activate intracellular transducer G proteins. Over 800 GPCRs in five main families have been identified in eukaryotes on the basis of genomic sequence analysis1,2. These receptors can be activated by hormones, neurotransmitters, odorants, light, or pheromones. Whereas the endogenous agonist is remains unclear or in dispute2.

GPCRs mediated the signaling of extracellular stimuli to intracellular responses via activation of heterotrimeric G proteins and their subsequent interaction with effector proteins. GPCRs have been implicated in a number of physiological functions including development of anxiety, behavior, homeostasis, cognition, appetite, and drug addiction3.

The GPCR family contains a diverse group of cell-surface mediators of signal transduction, its size exceeding other cell-surface protein receptors including the tyrosine kinase receptors, guanylyl cyclase receptors and ligand-gated ion channels4.

GPR108 (LUSTR2), has a large extracellular domain of an amino-terminal hydrophobic signal peptide sequence, and a carboxy-terminal seven-transmembrane domain5. Previous studies showed that GPR108 is linked to nuclear factor κB (NF-κB) signaling6 and regulates and inflammatory immune response and cancer signaling7.

Recently, GPR108 has been identified as a highly conserved cellular entry factor for Adeno-associated virus (AAV), a widely used and clinically approved gene therapy vector8.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 19/06/2022

Anti-GPR108 (extracellular) Antibody (#AGR-088) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize GPR108 from mouse, rat and human samples.

For research purposes only, not for human use
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