Anti-GPR120/FFAR4 (extracellular) Antibody

G-protein coupled receptor 120, Free fatty acid receptor 4, FFA4 Receptor, G-protein coupled receptor GT01, Omega-3 fatty acid receptor 1, O3far1
  • New
Cat #: AFR-014
Alternative Name G-protein coupled receptor 120, Free fatty acid receptor 4, FFA4 Receptor, G-protein coupled receptor GT01, Omega-3 fatty acid receptor 1, O3far1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide (C)RTHFPFFSDVKGDHR, corresponding to amino acid residues 22-36 of mouse FFAR4 (Accession Q7TMA4). Extracellular, N-terminus.
    Accession (Uniprot) Number Q7TMA4
    Gene ID 107221
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat – identical; human – 14/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic, ih, ip, lci
      • Western blot analysis of rat lung (lanes 1 and 3), rat tongue (lanes 2 and 4), human U-87 astrocytoma cells (lanes 5 and 7) and mouse MS1 endothelial cells (lanes 6 and 8) lysates:
        1,2,5,6. Anti-GPR120/FFAR4 (extracellular) Antibody (#AFR-014), (1:200).
        3,4,7,8. Anti-GPR120/FFAR4 (extracellular) Antibody, preincubated with the control peptide antigen.
        Western blot analysis of mouse colon (lanes 11 and 13) and human colorectal adenocarcinoma (Colo 205) cell line (lanes 12 and 14) lysates:
        9,10. Anti-GPR120/FFAR4 (extracellular) Antibody (#AFR-014), (1:200).
        11,12. Anti-GPR120/FFAR4 (extracellular) Antibody, preincubated with the control peptide antigen.
      • Cell surface detection of FFAR4 in live intact human THP-1 monocytic leukemia cells:
        ___ Cells.
        ___ Cells + goat anti-rabbit-FITC.
        ___ Cells + Anti-GPR120/FFAR4 (extracellular) Antibody (#AFR-014), (2.5 μg/0.5x106 cells) + goat anti-rabbit-FITC.
      • Free fatty acid receptor 4 (FFAR4, GPR120), is a member of the rhodopsin family of 7-transmembrane domain G-protein coupled receptors (GPCRs). GPR120/FFAR4 is activated by long chain fatty acids. The protein is a potential therapeutic target for the treatment of type 2 diabetes mellitus (T2DM). GPR120 is associated to insulin sensitizing, anti-inflammatory, and fat metabolism. Evidence shows that GPR120 may be involved in the development of obesity in mice and humans1-4. GPR120 regulates various physiological processes, including gut hormone secretion, islet function, osteoclastogenesis, anti-inflammation, and adipogenesis2-4.

        GPR120 binds to omega-3 fatty acids and stabilizes the metabolic homeostasis through a cascade of physiological activities. GPR120 exerts its physiological effects through one of two pathways that involves either Gαq or β-arrestin2,3.

        GPR120 has been shown to be ubiquitously expressed and is primarily detected in the intestine, adipocytes, and pro-inflammatory macrophages1,2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 04/06/2019

    Anti-GPR120/FFAR4 (extracellular) Antibody (#AFR-014) is a highly specific antibody directed against an epitope of the mouse Free fatty acid receptor 4. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize FFAR4 from rat, mouse, and human samples.

    For research purposes only, not for human use