Free shipping starts now, no minimum, no coupons required!

Anti-GPR120/FFAR4 (extracellular)-FITC Antibody

G-protein coupled receptor 120, Free fatty acid receptor 4, FFA4 Receptor, G-protein coupled receptor GT01, Omega-3 fatty acid receptor 1, O3far1
Cat #: AFR-014-F
Alternative Name G-protein coupled receptor 120, Free fatty acid receptor 4, FFA4 Receptor, G-protein coupled receptor GT01, Omega-3 fatty acid receptor 1, O3far1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RTHFPFFSDVKGDHR, corresponding to amino acid residues 22-36 of mouse FFAR4 (Accession Q7TMA4). Extracellular, N-terminus.
Accession (Uniprot) Number Q7TMA4
Gene ID 107221
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – identical; human – 14/15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Label Fluorescein isothiocyanate (FITC)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #AFR-014), and direct flow cytometry (labeled antibody).
Applications: fc
May also work in: ic*, lci*
Direct flow cytometry
  • Cell surface detection GPR120/FFAR4 in live intact mouse BV-2 microglia cells:
    Cell surface detection GPR120/FFAR4 in live intact mouse BV-2 microglia cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-GPR120/FFAR4 (extracellular)-FITC Antibody (#AFR-014-F), (2.5 µg).
  • Cell surface detection GPR120/FFAR4 in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection GPR120/FFAR4 in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-GPR120/FFAR4 (extracellular)-FITC Antibody (#AFR-014-F), (2.5 µg).
References
  1. Cox, J.M. et al. (2016) ACS Med. Chem. Lett. 8, 49.
  2. Chen, Y.L. et al. (2018) Mol. Med. 24, 60.
  3. Chen, Y. et al. (2018) Arthritis Res. Ther. 20, 163.
  4. Vallée Marcotte, B. et al. (2017) J. Pers. Med. 7, 4.
Scientific background

Free fatty acid receptor 4 (FFAR4, GPR120), is a member of the rhodopsin family of 7-transmembrane domain G-protein coupled receptors (GPCRs). GPR120/FFAR4 is activated by long chain fatty acids. The protein is a potential therapeutic target for the treatment of type 2 diabetes mellitus (T2DM). GPR120 is associated to insulin sensitizing, anti-inflammatory, and fat metabolism. Evidence shows that GPR120 may be involved in the development of obesity in mice and humans1-4. GPR120 regulates various physiological processes, including gut hormone secretion, islet function, osteoclastogenesis, anti-inflammation, and adipogenesis2-4.

GPR120 binds to omega-3 fatty acids and stabilizes the metabolic homeostasis through a cascade of physiological activities. GPR120 exerts its physiological effects through one of two pathways that involves either Gαq or β-arrestin2,3.

GPR120 has been shown to be ubiquitously expressed and is primarily detected in the intestine, adipocytes, and pro-inflammatory macrophages1,2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Check out more of our new products.

Last update: 23/08/2020

Anti-GPR120/FFAR4 (extracellular) Antibody (#AFR-014) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize FFAR4 from rat, mouse, and human samples.

Anti-GPR120/FFAR4 (extracellular)-FITC Antibody (#AFR-014-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

For research purposes only, not for human use
Shipping and Ordering information