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Anti-GPR139 Antibody

G Protein-Coupled Receptor 139, PGR3, G(Q)-Coupled Orphan Receptor GPRg1, GPRG1

Cat #: AGR-069
Alternative Name G Protein-Coupled Receptor 139, PGR3, G(Q)-Coupled Orphan Receptor GPRg1, GPRG1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RKSNFRLRGYSTGK, corresponding to amino acid residues 204 - 217 of mouse GPR139 (Accession Q80UC8). Intracellular, 3rd loop.
Accession (Uniprot) Number Q80UC8
Gene ID 209776
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, Human - identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):
    1-2. Anti-GPR139 Antibody (#AGR-069), (1:800).
    3-4. Anti-GPR139 Antibody, preincubated with GPR139 Blocking Peptide (BLP-GR069).
  • Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
    Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
    1. Anti-GPR139 Antibody (#AGR-069), (1:200).
    2. Anti-GPR139 Antibody, preincubated with GPR139 Blocking Peptide (BLP-GR069).
Immunohistochemistry
  • Expression of GPR139 in rat ventromedial hypothalamus (VMH).
    Expression of GPR139 in rat ventromedial hypothalamus (VMH).
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR139 Antibody (#AGR-069), (1:600), followed by goat anti-rabbit-AlexaFluor-488. A. GPR139 immunoreactivity (green) appears in tanycyte-like processes (arrows). B. Pre-incubation of the antibody with GPR139 Blocking Peptide (BLP-GR069), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = third ventricle.
References
  1. Rosenbaum, D.M. et al. (2009) Nature., 459, 356.
  2. Tang, X.I. et al. (2012) Acta. Pharmacol. Sin., 33, 363.
  3. Nøhr, A.C. et al. (2017) Neurochem. Int., 102, 105.
  4. Vedel, L. et al. (2020) Basic. Clin. Pharmacol. Toxicol., 126, 35.
Scientific background

G-protein-coupled receptors (GPCRs), are the largest family of membrane proteins and mediate most of the physiological responses to hormones, neurotransmitters and environmental stimulants. At the most basic level, all GPCRs are characterized by the presence of seven membrane-spanning α-helical segments separated by alternating intracellular and extracellular loop regions1. Orphan GPCRs are a group of GPCRs that their endogenous ligands remain unidentified and therefore their natural functions in doubt2.

GPR139 is an orphan GPCRs for which amino acids L-tryptophan and L-phenylalanine, the adrenocorticotropic hormone (ACTH), and α- and β-melanocyte-stimulating hormone have been proposed as endogenous agonists3. GPR139 was found to be highly conserved across species, as the amino acid sequence of the human GPR139 protein is 96%, 92% and 70% identical to the mouse, chicken and zebrafish orthologs, respectively. According to mRNA expression levels GPR139 is mainly expressed in the central nervous system (CNS). GPR139 has been reported to activate several G protein pathways of which Gq/11 is the primary one4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 15/11/2021

Anti-GPR139 Antibody (#AGR-069) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPR139 from rat, mouse and human samples.

For research purposes only, not for human use
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