- Peptide (C)KDIKEKSNVG(S)MEFK, corresponding to amino acid residues 155 - 169 of mouse GPR171 (Accession Q8BG55). 2nd extracellular loop.
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1, 2. Anti-GPR171 (extracellular) Antibody (#AGR-054), (1:200).
3, 4. Anti-GPR171 (extracellular) Antibody, preincubated with GPR171 (extracellular) Blocking Peptide (#BLP-GR054).
- Western blot analysis of human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):
- Cell surface detection of GPR171 in live intact human Jurkat T-cell leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-GPR171 (extracellular) Antibody (#AGR-054), (5 µg) + goat-anti-rabbit-FITC.
- Fricker, L.D. (2010) Mol. Biosyst. 6, 1355.
- Schöneberg, T. et al. (2007) Purinergic Signal. 3, 255.
- Wardman, J.H. et al. (2016) Sci. Signal. 9, ra55.
- Gomes, I. et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110, 16211.
- Bobeck, E.N. et al. (2017) Neuropsychopharmacology 42, 2527.
- McDermott, M.V. et al. (2019) J. Pharmacol. Exp. Ther. 371, 56
G protein-coupled receptor 171 (GPR171) is an orphan G-protein coupled receptor (GPCR) activated by BigLEN; one of the most abundant neuropeptides in the brain that is derived from ProSAAS1. GPR171 contains seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail. Its detailed structure and ligand binding domain structure was predicted by homology modeling based on its similarity to P2Y12 receptor2,3.
BigLEN–GPR171 neuropeptide-receptor system is expressed in the brain, at the basolateral amygdala (BLA) and the periaqueductal gray brain area4-6.
This system plays an important role in regulating feeding-related behaviors, food intake and body weight regulation. It is also involved in anxiety behavior and various psychiatric disorders4,5.
Furthermore, BigLEN-GPR171 are expressed in GABAergic neurons within the periaqueductal gray, a key brain area involved in pain modulation and opioid functions6.
GPR171 is a potential target for drug development in obesity and weight problems, anxiety, various psychiatric disorders and pain.
Species reactivity key:
Anti-GPR171 (extracellular) Antibody (#AGR-054) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and live cell flow cytometry. It has been designed to recognize GPR171 from human, rat, and mouse samples.
- Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020)
- Anti-P2Y12 Receptor (extracellular)-FITC Antibody (#APR-020-F)
- Anti-P2Y12 Receptor (extracellular)-PE Antibody (#APR-020-PE)
- Anti-P2Y12 Receptor Antibody (#APR-012)
- Anti-P2Y12 Receptor-ATTO Fluor-594 Antibody (#APR-012-AR)
- Anti-µ-Opioid Receptor (OPRM1) (extracellular) Antibody (#AOR-011)
- Anti-µ-Opioid Receptor (OPRM1) (extracellular)-ATTO Fluor-488 Antibody (#AOR-011-AG)
- Anti-µ-Opioid Receptor/OPRM1 (extracellular)-FITC Antibody (#AOR-011-F)
- Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#AGP-098)