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Anti-GPR171 (extracellular) Antibody

G-Protein Coupled Receptor 171, Platelet Activating Receptor Homolog, G-protein coupled receptor H963
Cat #: AGR-054
Alternative Name G-Protein Coupled Receptor 171, Platelet Activating Receptor Homolog, G-protein coupled receptor H963
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KDIKEKSNVG(S)MEFK, corresponding to amino acid residues 155 - 169 of mouse GPR171 (Accession Q8BG55). 2nd extracellular loop.
Accession (Uniprot) Number Q8BG55
Gene ID 229323
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – 14/15 amino acid residues identical; rat – 13/15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ih*, ip*, lci*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1, 2. Anti-GPR171 (extracellular) Antibody (#AGR-054), (1:200).
    3, 4. Anti-GPR171 (extracellular) Antibody, preincubated with GPR171 (extracellular) Blocking Peptide (#BLP-GR054).
  • Western blot analysis of human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):
    Western blot analysis of human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):
    1, 2. Anti-GPR171 (extracellular) Antibody (#AGR-054), (1:200).
    3, 4. Anti-GPR171 (extracellular) Antibody, preincubated with GPR171 (extracellular) Blocking Peptide (#BLP-GR054).
Indirect flow cytometry
  • Cell surface detection of GPR171 in live intact human Jurkat T-cell leukemia cells:
    Cell surface detection of GPR171 in live intact human Jurkat T-cell leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-GPR171 (extracellular) Antibody (#AGR-054), (5 µg) + goat-anti-rabbit-FITC.
References
  1. Fricker, L.D. (2010) Mol. Biosyst. 6, 1355.
  2. Schöneberg, T. et al. (2007) Purinergic Signal. 3, 255.
  3. Wardman, J.H. et al. (2016) Sci. Signal. 9, ra55.
  4. Gomes, I. et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110, 16211.
  5. Bobeck, E.N. et al. (2017) Neuropsychopharmacology 42, 2527.
  6. McDermott, M.V. et al. (2019) J. Pharmacol. Exp. Ther. 371, 56
Scientific background

G protein-coupled receptor 171 (GPR171) is an orphan G-protein coupled receptor (GPCR) activated by BigLEN; one of the most abundant neuropeptides in the brain that is derived from ProSAAS1. GPR171 contains seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail. Its detailed structure and ligand binding domain structure was predicted by homology modeling based on its similarity to P2Y12 receptor2,3.

BigLEN–GPR171 neuropeptide-receptor system is expressed in the brain, at the basolateral amygdala (BLA) and the periaqueductal gray brain area4-6.

This system plays an important role in regulating feeding-related behaviors, food intake and body weight regulation. It is also involved in anxiety behavior and various psychiatric disorders4,5.

Furthermore, BigLEN-GPR171 are expressed in GABAergic neurons within the periaqueductal gray, a key brain area involved in pain modulation and opioid functions6.

GPR171 is a potential target for drug development in obesity and weight problems, anxiety, various psychiatric disorders and pain.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

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Last update: 07/09/2020

Anti-GPR171 (extracellular) Antibody (#AGR-054) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and live cell flow cytometry. It has been designed to recognize GPR171 from human, rat, and mouse samples.

For research purposes only, not for human use
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