Anti-GPR34 (extracellular)-FITC Antibody

G-protein coupled receptor 34
  • New
Cat #: AGR-055-F
Alternative Name G-protein coupled receptor 34
  • Lyophilized Powder
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide CFHYRDRHNAKGE, corresponding to amino acid residues 197-209 of mouse GPR34 (Accession Q9R1K6). 2nd extracellular loop.
    • Anti-GPR34 (extracellular)-FITC Antibody
    Accession (Uniprot) Number Q9R1K6
    Gene ID 23890
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human, rat – 12/13 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label Fluorescein isothiocyanate (FITC)
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGR-055), and direct flow cytometry (labeled antibody).
    Applications: fc
    May also work in: ic*, lci*
    Direct flow cytometry
    • Anti-GPR34 (extracellular)-FITC Antibody
      Cell surface detection of GPR34 in live intact mouse BV-2 microglia cells:

      ___ Cells.
      ___ Cells + rabbit IgG isotype control-FITC.
      ___ Cells + Anti-GPR34 (extracellular)-FITC Antibody (#AGR-055-F), (2.5 µg).

    • Anti-GPR34 (extracellular)-FITC Antibody
      Cell surface detection of GPR34 in live intact human THP-1 monocytic leukemia cells:
      ___ Cells.
      ___ Cells + rabbit IgG isotype control-FITC.
      ___ Cells + Anti-GPR34 (extracellular)-FITC Antibody (#AGR-055-F), (2.5 µg).
    References
    1. Spicer, L.J. et al. (2017) Domest. Anim. Endocrinol. 59, 90.
    2. Schöneberg, T. et al. (2018) Pharmacol. Ther.189, 71.
    3. Sayama, M. et al. (2017) J. Med. Chem. 60, 6384.
    4. Jäger, E. et al. (2016) J. Immunol. 196, 2504.
    Scientific background

    The G-protein-coupled receptor 34 (GPR34) is currently classified as an orphan receptor of the P2Y12-like receptor group. The receptor belongs to the super family of G-protein coupled receptors (GPCRs)1.

    The N-terminus of human GPR34 has three putative N-glycosylation sites (NXS/T), and the C-terminus has a cysteine residue which is palmitoylated in many GPCRs. GPR34 contains several putative phosphorylation sites primed by a serine or threonine in the C-terminus2.

    GPR34 is highly expressed in immune cells such as mast cells, macrophages, microglia, and immune system–derived cell lines (HL-60, K562, WEHI-3B, RAW 264.7, P815). Studies show that GPR34 function is required to arrest microglia in the M0 homeostatic non-phagocytic phenotype and downregulation of GPR34 promotes the neurodegenerative microglia phenotype2,3,4.

    GPR34 is linked to several human diseases including immunological diseases and cancer. Most known effects of GPR34 are immune function-related. For example, GPR34 is significantly down-regulated in Mycobacterium tuberculosis-infected macrophages; GPR34 mRNA is significantly lower in the cerebrospinal fluid from multiple sclerosis patients; GPR34 is significantly upregulated in the striatum and cortex of patients with Parkinson's disease but downregulated in substantia nigraelevated GPR34 also plays a role in the progression of some tumors, such as human gastric adenocarcinoma, mucosa-associated lymphoid tissue (MALT) lymphoma, and colorectal cancer1-3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 11/02/2020

    Anti-GPR34 (extracellular) Antibody (#AGR-055) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize GPR34 from human, mouse, and rat samples.

    Anti-GPR34 (extracellular)-FITC Antibody (#AGR-055-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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