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Anti-GPR37L1 (extracellular) Antibody

G-Protein Coupled Receptor 37-Like 1, Endothelin B Receptor-Like Protein 2, ETBRLP2, Prosaposin Receptor GPR37L1

Cat #: AGR-050
Alternative Name G-Protein Coupled Receptor 37-Like 1, Endothelin B Receptor-Like Protein 2, ETBRLP2, Prosaposin Receptor GPR37L1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)EYPKPIHPAGLQPTK, corresponding to amino acid residues 66 - 80 of rat GPR37L1 (Accession Q9QYC5). Extracellular, N-terminus.
Accession (Uniprot) Number Q9QYC5
Gene ID GPR37L1
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical; human – 14 out of 15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*
Western blot
  • Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1-2. Anti-GPR37L1 (extracellular) Antibody (#AGR-050), (1:200). 3-4. Anti-GPR37L1 (extracellular) Antibody, preincubated with GPR37L1 (extracellular) Blocking Peptide (BLP-GR050).
  • Western blot analysis of human U-87 MG glioblastoma cell line lysate:
    Western blot analysis of human U-87 MG glioblastoma cell line lysate:
    1. Anti-GPR37L1 (extracellular) Antibody (#AGR-050), (1:200). 2. Anti-GPR37L1 (extracellular) Antibody, preincubated with GPR37L1 (extracellular) Blocking Peptide (BLP-GR050).
Immunohistochemistry
  • Expression of GPR37L1 in rat cerebellum.
    Expression of GPR37L1 in rat cerebellum.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR37L1 (extracellular) Antibody (#AGR-050), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. GPR37L1 immunoreactivity (green) appeared around Purkinje cell soma (arrows) and in the molecular layer (M). B. Pre-incubation of the antibody with GPR37L1 (extracellular) Blocking Peptide (BLP-GR050), suppressed staining. Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of GPR37L1 by indirect flow cytometry in live intact mouse BV-2 microglia cell line:
    Cell surface detection of GPR37L1 by indirect flow cytometry in live intact mouse BV-2 microglia cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-GPR37L1 (extracellular) Antibody (#AGR-050), (2.5µg) + goat-anti-rabbit-FITC.
References
  1. Nicola J. Smith (2015), Frontiers in Pharmacology, 6, 275.
  2. Donohue et al, (1998), Mol. Brain Res., 54, 152.
  3. Meyer et al, (2013), Proc Natl Acad Sci, 110, 9529.
  4. Zeng et al, (1997), Biochem Biophys Res Commun., 233, 559.
  5. Zheng et al, (2019), Am J Physiol Renal Physiol., 316, F506.
Scientific background

G prtoein coupled receptor 37L1 (GPR37L1), also known as Endothelin B Receptor-Like Protein 2, is an orphan receptor (a receptor without an assigned ligand yet) that shares structural homology with the endothelin receptors family of GPCRs. 1

GPR37L1 is a 481 amino acid GPCR with 68% similarity and 48% identity to GPR37, another orphan endothelin receptor like GPR. In addition to the seven trans- membrane helixes typical for GPCRs, it has a cysteine cluster at the end of helix 8. 2

GPR37L1 has been shown to bind the neuroprotective and glioprotective factor prosaposin (PSAP), although later studies did not confirm prosaposin as a functional ligand. 3

GPR37L1 is highly expressed in the central nervous system, with some expression in the spinal cord, placenta, liver, stomach, and testis. 4,5

GPR37L1 signaling pathway was shown to have an effect on motor activity, blood pressure regulation, cellular development and protection from cytotoxic agents. Deletion of the receptor caused marked developmental changes in the cerebellum, and hence the discovery of the native ligand for this receptor and studying the signaling pathway that is induced by it, is of high medicinal importance 1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

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Last update: 24/01/2021

Anti-GPR37L1 (extracellular) Antibody (#AGR-050) is a highly specific antibody directed against an extracellular epitope of the rat GPR37L1. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize GPR37L1 from mouse, rat and human samples.

For research purposes only, not for human use
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