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Anti-GPR39 (extracellular) Antibody

G-protein coupled receptor 39, Zinc receptor GPR39

Cat #: AGR-045
Alternative Name G-protein coupled receptor 39, Zinc receptor GPR39
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KPKHDWTRTYFR, corresponding to amino acid residues 309-320 of mouse GPR39 (Accession Q5U431). 3rd extracellular loop.
Accession (Uniprot) Number Q5U431
Gene ID 71111
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human – 11/12 amino acid residues identical; rat – 9/12 amino acid residues identical.
RRID AB_2756635.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, lci, wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of mouse kidney (lanes 1 and 3) and brain (lanes 2 and 4) lysates:
    Western blot analysis of mouse kidney (lanes 1 and 3) and brain (lanes 2 and 4) lysates:
    1,2 Anti-GPR39 (extracellular) Antibody (#AGR-045), (1:200).
    3,4. Anti-GPR39 (extracellular) Antibody, preincubated with GPR39 (extracellular) Blocking Peptide (#BLP-GR045).
  • Western blot analysis of rat colon (lanes 1 and 3) and pancreas (lanes 2 and 4) lysates:
    Western blot analysis of rat colon (lanes 1 and 3) and pancreas (lanes 2 and 4) lysates:
    1,2 Anti-GPR39 (extracellular) Antibody (#AGR-045), (1:200).
    3,4. Anti-GPR39 (extracellular) Antibody, preincubated with GPR39 (extracellular) Blocking Peptide (#BLP-GR045).
  • Western blot analysis of human HT-29 colorectal adenocarcinoma (lanes 1 and 3) and human MEG-01 megakaryoblastic leukemia (lanes 2 and 4) cell line lysates:
    Western blot analysis of human HT-29 colorectal adenocarcinoma (lanes 1 and 3) and human MEG-01 megakaryoblastic leukemia (lanes 2 and 4) cell line lysates:
    1,2 Anti-GPR39 (extracellular) Antibody (#AGR-045), (1:200).
    3,4. Anti-GPR39 (extracellular) Antibody, preincubated with GPR39 (extracellular) Blocking Peptide (#BLP-GR045).
Indirect flow cytometry
  • Cell surface detection of GPR39 by indirect flow cytometry in live intact human MEG-01 megakaryocytic leukemia cells: 
    Cell surface detection of GPR39 by indirect flow cytometry in live intact human MEG-01 megakaryocytic leukemia cells: 
    ___ Cells
    ___ Cells + goat-anti-rabbit-FITC 
    ___ Cells Anti-GPR39 (extracellular) Antibody(#AGR-045), (2.5μg) + goat-anti-rabbit-FITC
References
  1. Popovics, P. and Stewart, A.J. (2011) Cell. Mol. Life Sci. 68, 85.
  2. Mlyniec, K. et al. (2015) Int. J. Neuropsychopharmacol. 18, pyu002.
Scientific background

GPR39 (G-protein coupled receptor 39) is member of the rhodopsin-like family of 7-transmembrane containing GPCRs and is closely related to the ghrelin/neurotensin receptor subfamily.

The human GPR39 gene encodes the full-length receptor (GPR39-1a). GPR39 is expressed in various tissues including adipose tissue, heart and thyroid but is most abundant in the pancreas, GI tract, liver and kidney. 

The GPR39 receptor is comprised of an extracellular N-terminus, seven membrane spanning loops, and an intracellular C-terminus. It also contains four cysteine residues in its extracellular domains. These have been shown to form two disulfide bridges which are conserved within all vertebrate GPR39 sequences with the exception that the first bridge is absent from the fish receptors.

Although GPR39 has a high degree of constitutive activity mediated through Gαq and Gα12/13 pathways, it has been discovered recently that Zn2+ serves as a ligand capable of activating the receptor. Following Zinc activation, both pathways are significantly enhanced and signaling occurs through the Gαs pathway as well.

GPR39 has been implicated in several pathologies: Disruption of the GPR39 gene is associated with impaired insulin secretion. GPR39 knockout mice exhibit decreased insulin levels and lower levels of insulin promoting factor-1 and HNF-1α in pancreatic islets. GPR39 knockout mice also exhibit lower levels of CREB and BDNF proteins that are important in neuronal plasticity and antidepressant response thus leading to depressive-like behaviors in animals.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 06/11/2022

Anti-GPR39 (extracellular) Antibody (#AGR-045) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize GPR39 from human, mouse, and rat samples.

For research purposes only, not for human use
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