Anti-GPR55 (extracellular) Antibody

G-Protein Coupled Receptor 55
  • New
Cat #: ACR-062
Alternative Name G-Protein Coupled Receptor 55
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    • Peptide (C)RNRFILD(S)RMKQGIS, corresponding to amino acid residues 261 - 275 of mouse GPR55 (Accession Q3UJF0). 3rd extracellular loop.
    • Anti-GPR55 (extracellular) Antibody
    Accession (Uniprot) Number Q3UJF0
    Gene ID 227326
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – 14/15 amino acid residues identical; rat – 11/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG
    Specificity Not recommended for use with human samples.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic*, ih*, ip*, lci*
    Western blot
    • Anti-GPR55 (extracellular) Antibody
      Western blot analysis of rat brain membranes (lanes 1 and 3), and mouse brain membranes (lanes 2 and 4):
      1, 2. Anti-GPR55 (extracellular) Antibody (#ACR-062), (1:500).
      3, 4. Anti-GPR55 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-GPR55 (extracellular) Antibody
      Western blot analysis of mouse BV-2 microglia cell line lysate:
      1. Anti-GPR55 (extracellular) Antibody (#ACR-062), (1:500).
      2. Anti-GPR55 (extracellular) Antibody, preincubated with the negative control antigen.
    Indirect flow cytometry
    • Anti-GPR55 (extracellular) Antibody
      Cell surface detection of GPR55 in live intact mouse BV-2 microglia cells:
      ___ Cells.
      ___ Cells + goat-anti-rabbit-FITC.
      ___ Cells + Anti-GPR55 (extracellular) Antibody (#ACR-062), (2.5 µg) + goat-anti-rabbit-FITC.
    1. Mackie, K. and Stella, N. (2006) AAPS J. 8, 298.
    2. Lauckner, J.E. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105, 2699.
    3. Hill, J.D. et al. (2018) Br. J. Pharmacol. 175, 3407.
    4. Shore, D.M. and Reggio, P.H. (2015) Front. Pharmacol. 6, 69.
    5. Sharir, H. and Abood, M.E. (2010) Pharmacol. Ther. 126, 301.
    Scientific background

    G protein coupled receptor 55 (GPR55) is a cannabinoid receptor activated by a number of endo‐, phyto‐ and synthetic cannabinoid ligands such as THC, JWH015 and anandamide1-3.

    GPR55 is only 13.5% identical to the canonic cannabinoid receptor CB1 and 14.4% identical to CB2. Upon ligand binding, it initiates a signaling cascade quite distinct from those used by CB1 and CB2 that eventually leads to an increase in intracellular calcium levels. 

    GPR55 belongs to the Class A GPCRs. It shares many similarities with rhodopsin and as all other G-protein coupled receptors in its basic structure of seven transmembrane domains, intracellular C- terminus and extracellular N-terminal extensions4. The protein is highly expressed in large dorsal root ganglion neurons and its mRNA is present in the brain and periphery1.

    GPR55 has a role in self‐renewing adult neural stem cell development and in hippocampal neurogenesis3. Experiments with mice in which GPR55 has been inactivated reveal a role for this receptor in neuropathic and inflammatory pain as well as in bone physiology. Thus deciphering the pharmacology of this receptor could lead to new therapeutics in these fields5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 07/06/2020

    Anti-GPR55 (extracellular) Antibody (#ACR-062) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR55 from human, rat, and mouse samples. The antibody will not recognize the human protein.

    For research purposes only, not for human use
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