Anti-GPR56 (extracellular)-FITC Antibody

G-protein coupled receptor 56, Adhesion G-protein coupled receptor G1, ADGRG1
  • New
Cat #: AGR-047-F
Alternative Name G-protein coupled receptor 56, Adhesion G-protein coupled receptor G1, ADGRG1
  • Lyophilized Powder
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)RHTGRLHLRYGKND, corresponding to amino acid residues 86-109 of rat GPR56 (Accession Q8K3V3). Extracellular, N-terminus.
        Anti-GPR56 (extracellular)-FITC Antibody
    Accession (Uniprot) Number Q8K3V3
    Gene ID 260326
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – 13/14 amino acid residues identical; human – 11/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity This antibody recognizes the N-terminal fragment (GPR56-NT), that can be secreted to the media.
    Label Fluorescein isothiocyanate (FITC).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot

    Western blot analysis (unlabeled antibody, #AGR-047), and direct flow cytometry (labeled antibody).

    Applications: fc
    May also work in: ic*, lci*
      • Anti-GPR56 (extracellular)-FITC Antibody
        Cell surface detection of GPR56 in live intact mouse BV-2 microglia cells:
        ___ Cells.
        ___ Cells + rabbit IgG isotype control-FITC.
        ___ Cells + Anti-GPR56 (extracellular)-FITC Antibody (#AGR-047-F), (2.5 µg).
        Anti-GPR56 (extracellular)-FITC Antibody
        Cell surface detection of GPR56 in live intact human HL-60 promyelocytic leukemia cells:
        ___ Cells.
        ___ Cells + rabbit IgG isotype control-FITC.
        ___ Cells + Anti-GPR56 (extracellular)-FITC Antibody (#AGR-047-F), (5 µg).
    References
    • 1. Moreno, M. et al. (2017) Cell Rep. 21, 2183.
    • 2. Aust, G. et al. (2016) Handb. Exp. Pharmacol. 234, 369.
    • 3. Singer, K. et al. (2013) Mol. Neurobiol. 47, 186.
      • GPR56 (ADGRG1) is a member of the adhesion G-protein coupled receptor (GPCR) family, responsible for the regulation of cell adhesion, migration, polarity, and guidance. GPR56 levels are increased in many cancer types and seems to be relevant for tumor cell migration and invasion1,2. GPR56 structure is characterized by a long extracellular domain that is involved in cell-to-cell and cell-to-extracellular matrix interactions. The receptor contains a GPS motif, responsible for cleaving the protein. Seven different glycosylation sites along its N-terminal fragment are present1-3.

        Expression of Gpr56 mRNA can be found in neuronal progenitor cells of the cerebral cortical ventricular and subventricular zones during periods of neurogenesis. Studies show that GPR56 protein knockdown promotes mesenchymal differentiation of glioma stem-like initiating cells, accompanied by increased radioresistance in vitro and in vivo1-3.

        Mutations in GPR56 may cause bilateral frontoparietal polymicrogyria, a cobblestone-like cortical malformation, characterized by overmigrating neurons and the formation of neuronal ectopias on the surface of the brain2,3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 17/09/2019

    Anti-GPR56 (extracellular) Antibody (#AGR-047) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect flow cytometry applications. It recognizes an extracellular epitope and can potentially detect the protein in living cells. It has been designed to recognize ADGRG1 from rat, mouse, and human samples. The antibody recognizes the N-terminal fragment (GPR56-NT) that can be secreted to the media.

    Anti-GPR56 (extracellular)-FITC Antibody (#AGR-047-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

    For research purposes only, not for human use