Anti-GPR81/HCAR1 Antibody

Hydroxycarboxylic acid receptor 1, HCA1 Receptor, G-protein coupled receptor 81, GPR81, G Protein-Coupled Receptor 104, LACR1
  • New
Cat #: AHR-011
Alternative Name Hydroxycarboxylic acid receptor 1, HCA1 Receptor, G-protein coupled receptor 81, GPR81, G Protein-Coupled Receptor 104, LACR1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    Immunogen
    • Peptide CSKSSIDGANRSQR, corresponding to amino acid residues 319-332 of mouse GPR81 (HCAR1) (Accession Q8C131). Intracellular, C-terminus.
    Accession (Uniprot) Number Q8C131
    Gene ID 243270
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat – 9/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG
    Specificity The antibody will not recognize human GPR81 (HCAR1).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
    Western blot
    • Anti-GPR81/HCAR1 Antibody
      Western blot analysis of rat kidney (lanes 1 and 3) and rat liver (lanes 2 and 4) lysates:
      1, 2. Anti-GPR81/HCAR1 Antibody (#AHR-011), (1:200).
      3, 4. Anti-GPR81/HCAR1 Antibody, preincubated with the negative control antigen.
    • Anti-GPR81/HCAR1 Antibody
      Western blot analysis of mouse kidney lysate (lanes 1 and 3) and mouse liver membrane (lanes 2 and 4):
      1, 2. Anti-GPR81/HCAR1 Antibody (#AHR-011), (1:200).
      3, 4. Anti-GPR81/HCAR1 Antibody, preincubated with the negative control antigen.
    References
    1. Liu, C. et al. (2009) J. Biol. Chem. 284, 2811.
    2. Wallenius, K. et al. (2017) JCI Insight 2, e92564.
    3. Li, G. et al. (2014) PLoS One 9, e93041.
    4. Pu, J. et al. (2011) J. Lipid. Res. 52, 1319.
    5. Roland, C.L. et al. (2014) Cancer Res. 74, 5301.
    6. Kuei, C. et al. (2011) Mol. Pharmacol. 80, 848.
    Scientific background

    GPR81 is an orphan G-protein-coupled receptor that belongs to a family of hydroxy-carboxylic acid (HCA) receptors. Its endogenous ligand is L-lactate, a metabolite produced when glycolysis exceeds the capacity to oxidize pyruvate1,2.

    In addition to being a by-product of metabolism, lactate plays a role in insulin signaling, particularly in insulin mediated anti-lipolytic effects. Likewise, GPR81 is mostly expressed in adipose tissues and its activation results in inhibition of lipolysis and reduced hydrolysis of triglycerides to glycerol and fatty acids, suggesting that GPR81 may be an attractive drug discovery target for metabolic disorders4.

    Beyond the control of adipocyte lipolysis, GPR81 is also involved in neuronal signaling, regulation of blood flow to the retina. The receptor is overexpressed in several cancer types where it appears to play a role in the adaptation to the ischemic tumor microenvironment5.

    GPR81, like all other G protein coupled receptors, consists of seven transmembrane domains, in addition it possesses a highly conserved Cys-Glu-Ser-Phe motif in the second extracellular loop, essential for its function and six Cys residues at the extracellular domains that potentially form three disulfide bonds6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

    Check out more of our new products.

    Last update: 21/06/2020

    Anti-GPR81/HCAR1 Antibody (#AHR-011) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize HCAR1 from rat and mouse samples. The antibody will not recognize human GPR81.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Shipping and Ordering information