Anti-GPR83 (extracellular) Antibody

G-protein coupled receptor 83, G-protein coupled receptor 72, GPR72, Glucocorticoid induced receptor, GIR
  • New
Cat #: AGR-053
Alternative Name G-protein coupled receptor 83, G-protein coupled receptor 72, GPR72, Glucocorticoid induced receptor, GIR
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)RRYGAESQNPTVK, corresponding to amino acid residues 59-71 of mouse GPR83 (Accession P30731). Extracellular, N-terminus.
    • Anti-GPR83 (extracellular) Antibody
    Accession (Uniprot) Number P30731
    Gene ID 14608
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human, rat – identical
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic*, ih*, ip*, lci*
    Western blot
    • Anti-GPR83 (extracellular) Antibody
      Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
      1, 2. Anti-GPR83 (extracellular) Antibody (#AGR-053), (1:400).
      3, 4. Anti-GPR83 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-GPR83 (extracellular) Antibody
      Western blot analysis of human Jurkat T-cell leukemia cell line lysate:
      1. Anti-GPR83 (extracellular) Antibody (#AGR-053), (1:400).
      2. Anti-GPR83 (extracellular) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-GPR83 (extracellular) Antibody
      Expression of GPR83 in mouse hippocampus and cortex
      Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR83 (extracellular) Antibody (#AGR-053), (1:3000), followed by goat anti-rabbit-AlexaFluor-488. A. GPR83 staining (green) in hippocampal dentate gyrus is detected in hilar interneurons (vertical arrows) and around cells of the granule layer (horizontal arrows). H = hilus of dentate gyrus. G = granule layer. B. GPR83 immunoreactivity (green) in parietal cortex is observed in neurons of the pyramidal layer (vertical arrows) and in their apical dendrites (horizontal arrows). Cell nuclei are stained with DAPI (blue).
    Indirect flow cytometry
    • Anti-GPR83 (extracellular) Antibody
      Cell surface detection of GPR83 in live intact human Jurkat T-cell leukemia cells:
      ___ Cells.
      ___ Cells + goat-anti-rabbit-FITC.
      ___ Cells + Anti-GPR83 (extracellular) Antibody (#AGR-053), (5 µg) + goat-anti-rabbit-FITC.
    References
    1. Gomes, I. et al. (2016) Sci. Signal. 9, ra43.
    2. Cherezov, V. et al. (2007) Science 318, 1258.
    3. Khan, M.Z. and He, L. (2017) Psychopharmacology 24, 1181.
    4. Sugimoto, N. et al. (2006) Int. Immunol. 18, 1197.
    Scientific background

    GPR83 (JP05, GIR, GPR72) is a G-protein coupled receptor (GPCR) highly expressed in the brain, spleen and thymus, suggesting that this receptor may be targeted to treat neurological and immune disorders1.

    GPCR is a family of receptors responsible for initiation of a myriad of intracellular signaling cascades, these receptors are characterized by an extracellular N-terminus, intracellular C-terminal tail, and seven membrane-spanning α-helices (TM-1 to TM-7)2.

    In the brain GPR83 is expressed in the hippocampus, amygdala, prefrontal cortex, and in various hypothalamic nuclei where it may play a significant role in learning memory, reward, emotional behaviors, and stress regulation3. GPR83 is also expressed in regulatory T cells (Tregs) and it could potentially be used as a specific marker used to identify natural Tregs4.

    GPR83 was considered as an "orphan" GPCR. Recently, PEN, a neuropeptide was identified as its endogenous ligand1.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 18/03/2020

    Anti-GPR83 (extracellular) Antibody (#AGR-053) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize GPR83 from rat, mouse, and human samples.

    For research purposes only, not for human use
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