Anti-GPR84 (extracellular)-FITC Antibody

G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33
  • New
Cat #: AGR-052-F
Alternative Name G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33
  • Lyophilized Powder
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide CT(S)SFDRIRGRPYTT, corresponding to amino acid residues 166-180 of human GPR84 (Accession Q9NQS5). 2nd extracellular loop.
        Anti-GPR84 (extracellular)-FITC Antibody
    Accession (Uniprot) Number Q9NQS5
    Gene ID 53831
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human – 14/15 amino acid residues identical; mouse, rat – 13/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label Fluorescein isothiocyanate (FITC).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 15 μl or 50 µl double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGR-052), and direct flow cytometry (labeled antibody).
    Applications: fc
    May also work in: lci*
      • Anti-GPR84 (extracellular)-FITC Antibody
        Cell surface detection of GPR84 in live intact mouse BV-2 microglia cells:
        ___ Cells.
        ___ Cells + rabbit IgG isotype control-FITC.
        ___ Cells + Anti-GPR84 (extracellular)-FITC Antibody (#AGR-052-F), (5 µg).
        Anti-GPR84 (extracellular)-FITC Antibody
        Cell surface detection of GPR84 in live intact human THP-1 monocytic leukemia cells:
        ___ Cells.
        ___ Cells + rabbit IgG isotype control-FITC.
        ___ Cells + Anti-GPR84 (extracellular)-FITC Antibody (#AGR-052-F), (2.5 µg).
    References
      • GPR84 is an orphan G-protein coupled receptor (GPCR) activated by micromolar concentrations of medium-chain fatty acids (MCFAs). MCFAs that activate GPR84 are usually with carbon chain lengths of 9-14, which couples primarily to a pertussis toxin (PTX)-sensitive Gi/o pathway. The most potent agonists of GPR84 includes decanoic acid, undecanoic acid and lauric acid. GPR84 demonstrates an important role in inflammation, immunity, and cancer1-3.

        GPR84 is predominantly expressed in immune system-related tissues and cells, such as bone marrow, spleen, lung, lymph nodes and brain microglia, T-cells, macrophages and glial cells. Its expression is also detected in adipocytes.

        Studies have shown that GPR84 activation in murine macrophage-like cell line results in high levels of LPS-stimulated IL-12 p40 production. In addition, GPR84 knockout mice display increased Th2 cytokine production1-3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 17/10/2019

    Anti-GPR84 (extracellular) Antibody (#AGR-052) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize GPR84 from human, rat, and mouse samples.

    Anti-GPR84 (extracellular)-FITC Antibody (#AGR-052-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

    For research purposes only, not for human use