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Anti-GPR91 (SUCNR1) (extracellular)-FITC Antibody

G-protein coupled receptor 91, Succinate receptor 1
Cat #: ASR-090-F
Alternative Name G-protein coupled receptor 91, Succinate receptor 1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RNVRIASRLDSWPQG, corresponding to amino acid residues 248-262 of rat GPR91 (Accession Q6IYF9). 3rd extracellular loop.
Accession (Uniprot) Number Q6IYF9
Gene ID 408199
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – 14/15 amino acid residues identical; human – 12/15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label Fluorescein isothiocyanate (FITC).
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ASR-090), and direct flow cytometry (labeled antibody).
Applications: fc
May also work in: ic*, lci*
Direct flow cytometry
  • Cell surface detection of GPR91 in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of GPR91 in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-GPR91 (SUCNR1) (extracellular)-FITC Antibody (#ASR-090-F), (2.5 µg).
  • Cell surface detection of GPR91 in live intact mouse J774 macrophage cells:
    Cell surface detection of GPR91 in live intact mouse J774 macrophage cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-GPR91 (SUCNR1) (extracellular)-FITC Antibody (#ASR-090-F), (2.5 µg).
  • Cell surface detection of GPR91 in live intact human Jurkat T-cell leukemia cells:
    Cell surface detection of GPR91 in live intact human Jurkat T-cell leukemia cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-GPR91 (SUCNR1) (extracellular)-FITC Antibody (#ASR-090-F), (2.5 µg).
References
  1. Lu, Y.T. et al. (2018) Cell Death Dis. 9, 672.
  2. Cherif, H. et al. (2018) PLoS Biol. 16, e2003619.
  3. Le, C.T. et al. (2018) PLoS One 13, e0192146.
Scientific background

GPR91 (Succinate Receptor 1), a member of the GPCR superfamily, is specifically activated by extracellular succinate outside the tricarboxylic acid cycle, resulting in activation of paracrine and endocrine effectors. Activation of GPR91 is associated with several diseases, including hypertension and diabetes1,2.

GPR91 was shown to regulate normal retinal vascularization, proliferative ischemic retinopathy and cortical revascularization post-ischemia. Activation of GPR91 influences different tissues such as kidney, heart, retina, white adipose tissue, and liver.

Through the activation of GPR91, succinate affects motility, migration, and growth of dendritic cells. In addition, the receptor directly promotes chemotaxis and potentiates activation initiated by Toll-like receptor agonists in dendritic cells1-3.

In the heart GPR91 mediates succinate-induced cardiomyocyte death. It was demonstrated that upholding levels of serum succinate can cause cardiac hypertrophy through the activation of the GPR911,2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-GPR91 (SUCNR1) (extracellular) Antibody (#ASR-090) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and live cell flow cytometry. It has been designed to recognize GPR91 from rat, mouse, and human samples.

Anti-GPR91 (SUCNR1) (extracellular)-FITC Antibody (#ASR-090-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

For research purposes only, not for human use
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