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Anti-GPR97 (ADGRG3)(extracellular) Antibody

Adhesion G Protein-Coupled Receptor G3, G-protein coupled receptor 97

Cat #: AGR-057
Alternative Name Adhesion G Protein-Coupled Receptor G3, G-protein coupled receptor 97
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CNVENLQRYWLNYE, corresponding to amino acid residues 62 - 75 of human ADGRG3 (Accession Q86Y34). Extracellular, N-term.
Accession (Uniprot) Number Q86Y34
Gene ID 222487
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat – 12 out of 14 amino acid residues identical
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1-2. Anti-GPR97 (ADGRG3)(extracellular) Antibody (#AGR-057), (1:200).
    3-4. Anti-GPR97 (ADGRG3)(extracellular) Antibody, preincubated with GPR97 (ADGRG3)(extracellular) Blocking Peptide (BLP-GR057).
  • Western blot analysis of human THP-1 monocytic leukemia cell line lysate:
    Western blot analysis of human THP-1 monocytic leukemia cell line lysate:
    1. Anti-GPR97 (ADGRG3)(extracellular) Antibody (#AGR-057), (1:200).
    2. Anti-GPR97 (ADGRG3)(extracellular) Antibody, preincubated with GPR97 (ADGRG3)(extracellular) Blocking Peptide (BLP-GR057).
Immunohistochemistry
  • Expression of GPR97 in rat spinal cord.
    Expression of GPR97 in rat spinal cord.
    Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-GPR97 (ADGRG3)(extracellular) Antibody (#AGR-057), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the ventral horn of the spinal cord region, showed immunoreactivity of GPR97 (green) in neuronal outlines (arrows). B. Pre-incubation of the antibody with GPR97 (ADGRG3)(extracellular) Blocking Peptide (BLP-GR057), suppressed staining. Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of GPR97 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    Cell surface detection of GPR97 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-GPR97 (ADGRG3)(extracellular) Antibody (#AGR-057) (2.5μg) + goat-anti-rabbit-FITC.
References
  1. Bassilana, F. et al. (2019) Nat. Rev. Drug. Discov. 18, 869.
  2. Ping, YQ. et al. (2021) Nature. 589, 620.
  3. Hsiao, CC. et al. (2018) Front. Immunol. 9, 2830.
  4. Shi, J. et al. (2016) Sci. Rep. 6, 24649.
Scientific background

Adhesion G protein-coupled receptors (aGPCRs) are one of the five main families in the GPCR superfamily. They act as a molecular switch that regulate many physiological processes, including brain development, ion–water homeostasis, inflammation and cell-fate determination1.

One member of the aGPCRs family is the orphan receptor GPR97 (also known as ADGRG3). GPR97 consist of seven-transmembrane domain and a GPCR-Autoproteolysis INducing (GAIN) domain. Auto-cleavage via the GAIN domain produces two subunits: the α-subunit (N-terminal fragment (NTF)) and the β-subunit (C-terminal fragment) that remain non-covalently associated at the cell surface2.

GPR97 is mainly expressed in immune cells such as neutrophils, eosinophils, and mast cells. Activation of GPR97 results in reduction in cAMP levels in combination with an increase in downstream effectors of Gβγ, such as SRE and NF-κB3. GPR97 was found to regulate B-cell development in mice and antimicrobial activity in human granulocytes3. In addition, studies have shown that GPR97 is involved in macrophage inflammation in high fat diet–induced obesity in mice4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 15/08/2021

Anti-GPR97 (ADGRG3)(extracellular) Antibody (#AGR-057) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize GPR97 from rat, mouse and human samples.

For research purposes only, not for human use
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