Name: Anti-Histamine H1 Receptor (HRH1) Antibody
Brand: Alomone Labs
SKU: AHR-001

Cat #: AHR-001

Alternative Name H1R, HH1R

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

May also work in: h*

Immunogen

Peptide (C)HSRQYVSGLHLNRE, corresponding to amino acids 396-409 of rat HRH1 (Accession P31390). 3^rdintracellular loop.

Accession (Uniprot) Number P31390

Gene ID 24448

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - identical; human, dog - 13/14 amino acid residues identical.

RRID AB_2039915.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ifc, ih, wb

May also work in: ip*

Western blot

Rat heart membranes (1:200), rat basophilic leukemia cell lysates (1:200) and mouse brain membranes (1:400).
Western blot analysis of rat heart membranes (lanes 1 and 3) and rat basophilic leukemia (RBL) cell lysates (lanes 2 and 4):
1,2. Anti-Histamine H₁ Receptor (HRH1) Antibody (#AHR-001), (1:200).
3,4. Anti-Histamine H₁ Receptor (HRH1) Antibody, preincubated with Histamine H1 Receptor/HRH1 Blocking Peptide (#BLP-HR001).
Western blot analysis of mouse brain membranes:
1. Anti-Histamine H₁ Receptor (HRH1) Antibody (#AHR-001), (1:400).
2. Anti-Histamine H₁ Receptor (HRH1) Antibody, preincubated with Histamine H1 Receptor/HRH1 Blocking Peptide (#BLP-HR001).

Immunohistochemistry

Rat lung sections (paraffin-embedded), (1:100) and rat brain sections (frozen), (1:100).

Immunocytochemistry

Mouse isolated microglia (Apolloni, S. et al. (2017) Front. Immunol. 8, 1689.).

Indirect flow cytometry

Mouse P815 mast cells (Dong, H. et al. (2019) Front. Cell. Neurosci. 13, 191.).

Scientific background

Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.¹

The biological effects of histamine are mediated through four receptors (H₁-H₄ receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.

H₁ receptors couple to G_q/G₁₁ proteins leading to phospholipase C activation, inositol phosphate production and calcium mobilization.¹

H₁ receptors are expressed in many cell types including endothelial and smooth muscle cells where they mediate vasodilatation and increased vascular permeability effects. H1R is widely distributed through the brain where it mediates sleep-wake cycles and regulates cognitive functions. The receptor is expressed in several cell populations involved in the immune response, including T-cells and B-cells, monocytes and eosinophiles.^2,3

H₁ anti-histamine compounds have been available for almost thirty years and are among the most commonly used medication for conditions such as allergic inflammation. In addition, H₁ anti-histamines are also prescribed for the treatment of insomnia and analgesia.^2,3

Lyophilized Powder

Image & Title:

Anti-Histamine H1 Receptor (HRH1) Antibody

Expression of Histamine H₁ Receptor in mouse aortic root.Immunohistochemical staining of mouse aortic root sections using Anti-Histamine H₁ Receptor (HRH1) Antibody (#AHR-001). H₁R staining is shown in green.Adapted from Raveendran, V.V. et al. (2014) PLoS ONE 9, e10265. with permission of PLos.

Last update: 02/08/2022

For research purposes only, not for human use

Specifications

Citations

Applications

Scientific Background

Shipping and Ordering information

Control Product

Histamine H1 Receptor/HRH1 Blocking Peptide (#BLP-HR001) is the original antigen used for immunization during Anti-Histamine H₁ Receptor (HRH1) Antibody (#AHR-001) generation. The blocking peptide binds and ‘blocks’ Anti-Histamine H1 Receptor/HRH1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Histamine H1 Receptor/HRH1 Blocking Peptide

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Certificate of Analysis

Anti-Histamine H₁ Receptor (HRH1) Antibody

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Specifications

Citations

Applications

Scientific Background

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