Guinea pig Anti-HCN1 Antibody

Hyperpolarization-activated cyclic nucleotide-gated potassium channel 1, HAC2, Brain cyclic nucleotide-gated channel 1, BCNG1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
    Cat #: AGP-203
    Alternative Name Hyperpolarization-activated cyclic nucleotide-gated potassium channel 1, HAC2, Brain cyclic nucleotide-gated channel 1, BCNG1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Guinea pig
    Reactivity: r
    May also work in: h*, m*
    Immunogen
    • Peptide (C)KPNSASNSRDDGNSVYPSK, corresponding to amino acid residues 6-24 of rat HCN1 (Accession Q9JKB0). Intracellular, N-terminus.
    • Guinea pig Anti-HCN1 Antibody
    Accession (Uniprot) Number Q9JKB0
    Gene ID 84390
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 18/19 amino acid residues identical; human - 16/19 amino acid residues identical.
    RRID AB_2756625.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Guinea pig total IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
    Western blot
    • Guinea pig Anti-HCN1 Antibody
      Western blot analysis of rat brain lysate:
      1. Guinea pig Anti-HCN1 Antibody (#AGP-203), (1:500).
      2. Guinea pig Anti-HCN1 Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Guinea pig Anti-HCN1 Antibody
      Expression of HCN1 in rat cerebellum
      Immunohistochemical staining of rat cerebellum using Guinea pig Anti-HCN1 Antibody (#AGP-203). A. HCN1 staining (red) appears in the pinceau structures (arrows). B. DAPI is used as the counterstain (blue). C. Merged image of A and B.
    References
    1. Much, B. et al. (2003) J. Biol. Chem. 278, 43781.
    2. Notomi, T. et al. (2004) J. Comp. Neurol. 471, 241.
    3. Gravante, B. et al. (2004) J. Biol. Chem. 279, 43847.
    Scientific background

    Hyperpolarization-activated cation currents (Ih) appear in the heart and the brain and have a crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.1,2

    The Ih currents are generated by the hyperpolarization-activated cyclic nucleotide-gated channel family (HCN), which is comprised of four homologous members, HCN1-4.

    Each HCN subunit consists of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain for cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.2 The HCN subunits can form functional homomers and can also co-assemble into functional heteromers.2

    The channels are closely related to each other and share a homology of about 60%. However, their similarity decreases in the cytoplasmic N- and C-termini. The channels HCN1-4 mainly differ from each other in their speed of activation and the extent to which they are modulated by cAMP. HCN1, weakly affected by cAMP, is the fastest channel, followed by HCN2HCN3 and HCN4

    HCN1 is extensively expressed in the brain, in specific areas like the neocortex, hippocampus, cerebellum and superior colliculus.2,3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat HCN1 channel. Guinea pig Anti-HCN1 Antibody (#AGP-203) raised in guinea pig can be used in western blot and immunohistochemistry applications. It has been designed to recognize HCN1 from human, rat and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-HCN1 Antibody (#APC-056) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Western blot citations
    1. Rat brain and kidney lysate (1:500).
      Lopez-Gonzalez, Z. et al. (2016) Histochem. Cell. Biol. 145, 25.
    Immunohistochemistry citations
    1. Rat kidney sections (1:100).
      Lopez-Gonzalez, Z. et al. (2016) Histochem. Cell. Biol. 145, 25.
    Shipping and Ordering information